Exploiting sequence space: Shuffling in vivo formed complementarity determining regions into a master framework
Research output: Contribution to journal › Article
A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region. A synthetic gene library of soluble VH-fragments was created and the complexity of the library was determined by sequencing. The synthetic genes were diverse and contained random combinations of CDR from different germlines. All CDR were randomised in one step and by using in vivo formed CDR, the length, sequence and combination were varied simultaneously.
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|Number of pages||6|
|Publication status||Published - 1998 Jul 30|