Exploiting sequence space: Shuffling in vivo formed complementarity determining regions into a master framework

Research output: Contribution to journalArticle

Abstract

A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region. A synthetic gene library of soluble VH-fragments was created and the complexity of the library was determined by sequencing. The synthetic genes were diverse and contained random combinations of CDR from different germlines. All CDR were randomised in one step and by using in vivo formed CDR, the length, sequence and combination were varied simultaneously.

Details

Authors
Organisations
External organisations
  • BioInvent Therapeutic AB
Research areas and keywords

Keywords

  • CDR shuffling, Gene assembly, Immunoglobulin germline gene, Primer design, Synthetic gene library
Original languageEnglish
Pages (from-to)471-476
Number of pages6
JournalGene
Volume215
Issue number2
Publication statusPublished - 1998 Jul 30
Publication categoryResearch
Peer-reviewedYes