Exploiting sequence space: Shuffling in vivo formed complementarity determining regions into a master framework

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Exploiting sequence space : Shuffling in vivo formed complementarity determining regions into a master framework. / Jirholt, P.; Ohlin, M.; Borrebaeck, C. A K; Söderlind, E.

In: Gene, Vol. 215, No. 2, 30.07.1998, p. 471-476.

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TY - JOUR

T1 - Exploiting sequence space

T2 - Shuffling in vivo formed complementarity determining regions into a master framework

AU - Jirholt, P.

AU - Ohlin, M.

AU - Borrebaeck, C. A K

AU - Söderlind, E.

PY - 1998/7/30

Y1 - 1998/7/30

N2 - A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region. A synthetic gene library of soluble VH-fragments was created and the complexity of the library was determined by sequencing. The synthetic genes were diverse and contained random combinations of CDR from different germlines. All CDR were randomised in one step and by using in vivo formed CDR, the length, sequence and combination were varied simultaneously.

AB - A novel approach in molecular design is presented, where in vivo formed complementarity determining regions (CDR) from antibody genes were shuffled into a specific framework region. A synthetic gene library of soluble VH-fragments was created and the complexity of the library was determined by sequencing. The synthetic genes were diverse and contained random combinations of CDR from different germlines. All CDR were randomised in one step and by using in vivo formed CDR, the length, sequence and combination were varied simultaneously.

KW - CDR shuffling

KW - Gene assembly

KW - Immunoglobulin germline gene

KW - Primer design

KW - Synthetic gene library

UR - http://www.scopus.com/inward/record.url?scp=0032581610&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(98)00317-5

DO - 10.1016/S0378-1119(98)00317-5

M3 - Article

C2 - 9714846

AN - SCOPUS:0032581610

VL - 215

SP - 471

EP - 476

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -