Exploring biomarkers associated with deteriorating vascular health using a targeted proteomics chip: The SABPA study

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Exploring biomarkers associated with deteriorating vascular health using a targeted proteomics chip : The SABPA study. / Dieden, Anna; Malan, Leone; Mels, Catharina M C; Lammertyn, Leandi; Wentzel, Annemarie; Nilsson, Peter M; Gudmundsson, Petri; Jujic, Amra; Magnusson, Martin.

In: Medicine, Vol. 100, No. 20, e25936, 21.05.2021.

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Dieden, Anna ; Malan, Leone ; Mels, Catharina M C ; Lammertyn, Leandi ; Wentzel, Annemarie ; Nilsson, Peter M ; Gudmundsson, Petri ; Jujic, Amra ; Magnusson, Martin. / Exploring biomarkers associated with deteriorating vascular health using a targeted proteomics chip : The SABPA study. In: Medicine. 2021 ; Vol. 100, No. 20.

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TY - JOUR

T1 - Exploring biomarkers associated with deteriorating vascular health using a targeted proteomics chip

T2 - The SABPA study

AU - Dieden, Anna

AU - Malan, Leone

AU - Mels, Catharina M C

AU - Lammertyn, Leandi

AU - Wentzel, Annemarie

AU - Nilsson, Peter M

AU - Gudmundsson, Petri

AU - Jujic, Amra

AU - Magnusson, Martin

N1 - Copyright © 2021 the Author(s). Published by Wolters Kluwer Health, Inc.

PY - 2021/5/21

Y1 - 2021/5/21

N2 - ABSTRACT: In this observational study, by the use of a multiplex proteomic platform, we aimed to explore associations between 92 targeted proteins involved in cardiovascular disease and/or inflammation, and phenotypes of deteriorating vascular health, with regards to ethnicity.Proteomic profiling (92 proteins) was carried out in 362 participants from the Sympathetic activity and Ambulatory Blood Pressure in Africans (SABPA) study of black and white African school teachers (mean age 44.7 ± 9.9 years, 51.9% women, 44.5% Black Africans, 9.9% with known cardiovascular disease). Three proteins with <15% of samples below detectable limits were excluded from analyses. Associations between multiple proteins and prevalence of hypertension as well as vascular health [Carotid intima-media thickness (cIMT) and pulse wave velocity (PWV)] measures were explored using Bonferroni-corrected regression models.Bonferroni-corrected significant associations between 89 proteins and vascular health markers were further adjusted for clinically relevant co-variates. Hypertension was associated with growth differentiation factor 15 (GDF-15) and C-X-C motif chemokine 16 (CXCL16). cIMT was associated with carboxypeptidase A1 (CPA1), C-C motif chemokine 15 (CCL15), chitinase-3-like protein 1 (CHI3L1), scavenger receptor cysteine-rich type 1 protein M130 (CD163) and osteoprotegerin, whereas PWV was associated with GDF15, E-selectin, CPA1, fatty acid-binding protein 4 (FABP4), CXCL16, carboxypeptidase B (CPB1), and tissue-type plasminogen activator. Upon entering ethnicity into the models, the associations between PWV and CPA1, CPB1, GDF-15, FABP4, CXCL16, and between cIMT and CCL-15, remained significant.Using a multiplex proteomic approach, we linked phenotypes of vascular health with several proteins. Novel associations were found between hypertension, PWV or cIMT and proteins linked to inflammatory response, chemotaxis, coagulation or proteolysis. Further, we could reveal whether the associations were ethnicity-dependent or not.

AB - ABSTRACT: In this observational study, by the use of a multiplex proteomic platform, we aimed to explore associations between 92 targeted proteins involved in cardiovascular disease and/or inflammation, and phenotypes of deteriorating vascular health, with regards to ethnicity.Proteomic profiling (92 proteins) was carried out in 362 participants from the Sympathetic activity and Ambulatory Blood Pressure in Africans (SABPA) study of black and white African school teachers (mean age 44.7 ± 9.9 years, 51.9% women, 44.5% Black Africans, 9.9% with known cardiovascular disease). Three proteins with <15% of samples below detectable limits were excluded from analyses. Associations between multiple proteins and prevalence of hypertension as well as vascular health [Carotid intima-media thickness (cIMT) and pulse wave velocity (PWV)] measures were explored using Bonferroni-corrected regression models.Bonferroni-corrected significant associations between 89 proteins and vascular health markers were further adjusted for clinically relevant co-variates. Hypertension was associated with growth differentiation factor 15 (GDF-15) and C-X-C motif chemokine 16 (CXCL16). cIMT was associated with carboxypeptidase A1 (CPA1), C-C motif chemokine 15 (CCL15), chitinase-3-like protein 1 (CHI3L1), scavenger receptor cysteine-rich type 1 protein M130 (CD163) and osteoprotegerin, whereas PWV was associated with GDF15, E-selectin, CPA1, fatty acid-binding protein 4 (FABP4), CXCL16, carboxypeptidase B (CPB1), and tissue-type plasminogen activator. Upon entering ethnicity into the models, the associations between PWV and CPA1, CPB1, GDF-15, FABP4, CXCL16, and between cIMT and CCL-15, remained significant.Using a multiplex proteomic approach, we linked phenotypes of vascular health with several proteins. Novel associations were found between hypertension, PWV or cIMT and proteins linked to inflammatory response, chemotaxis, coagulation or proteolysis. Further, we could reveal whether the associations were ethnicity-dependent or not.

U2 - 10.1097/MD.0000000000025936

DO - 10.1097/MD.0000000000025936

M3 - Article

C2 - 34011069

VL - 100

JO - Medicine

JF - Medicine

SN - 1536-5964

IS - 20

M1 - e25936

ER -