Exploring the Substrate Specificity and Enantioselectivity of a Baeyer-Villiger Monooxygenase from Dietzia sp D5: Oxidation of Sulfides and Aldehydes

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Baeyer-Villiger monooxygenases (BVMOs) are valuable enzymes for specific oxyfunctionalization chemistry. They catalyze the oxidation of ketones to esters, but are also capable of oxidizing other chemical functions, namely aldehydes and heteroatoms such as sulfur, nitrogen, selenium and boron. The oxidation specificity and enantioselectivity of a newly characterized BVMO (BVMO4) from a strain of Dietzia towards sulfide- and aldehyde substrates have been studied. BVMO4 could react with sulfides containing an aromatic group. The presence of a substituent on the aromatic group was tolerated when they were in the meta- and para position and the oxidations yielded predominantly the (R)-sulfoxides. Similarly, BVMO4 displayed a higher activity for aldehydes containing a phenyl group, but long aliphatic aldehydes, namely octanal and decanal, were also accepted as substrate by this enzyme. The major oxidation products of the aldehyde substrates were the respective carboxylic acids in contrast to formate ester that was obtained in most of the previous reports. The Baeyer-Villiger oxidation of the substrate 2-phenylpropionaldehyde was studied in further detail and the corresponding acid product was obtained with good regio- and enantioselectivity. This is a unique feature for BVMO4 and is of great interest for further exploration of an alternative biocatalytic process.


  • Serena Bisagni
  • Benjamin Summers
  • Selin Kara
  • Rajni Hatti-Kaul
  • Gideon Grogan
  • Gashaw Mamo
  • Frank Hollmann
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology


  • Baeyer-Villiger monooxygenase, 2-Phenylpropionaldehyde, Dietzia, Profen, Biocatalysis
Original languageEnglish
Pages (from-to)366-375
JournalTopics in Catalysis
Issue number5
Publication statusPublished - 2014
Publication categoryResearch