Expression of lactate dehydrogenases A and B during chicken spermatogenesis: characterization of testis specific transcripts.

Research output: Contribution to journalArticle

Abstract

The substrates required for glycolysis change markedly at successive stages of spermatogenesis suggesting a considerable plasticity in the expression of glycolytic enzymes. Lactate dehydrogenase (LDH) isoenzymes, LDH-A and LDH-B, are expressed in premeiotic, meiotic cells, and early spermatids, both in avian and mammalian spermatogenesis. Highly polyadenylated forms, particularly of LDH-A, were detected in chicken testis. While mammals and columbid birds express the testis specific LDH-C gene in meiotic and postmeiotic cells, several LDH-B testis specific transcripts were detected in the corresponding cells during chicken spermatogenesis. These testis specific transcripts and the mRNA of mammalian LDH-C show several properties in common, such as temporal correlation of expression, mRNA stability, and repression of premature translation. These observations suggest that the testis specific transcripts could perform during chicken spermatogenesis the functions of the LDH-C mRNA in mammalian testis.

Details

Authors
  • Wilma Martinez Arias
  • C Mezquita
  • J Mezquita
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Basic Medicine

Keywords

  • * 5' Untranslated Regions * Amino Acid Sequence * Animals * Base Sequence * Chickens * DNA Primers * Isoenzymes * L-Lactate Dehydrogenase/genetics* * Male * Molecular Sequence Data * RNA, Messenger/genetics* * Spermatogenesis* * Testis/enzymology*
Original languageEnglish
Pages (from-to)15-27
JournalJournal of Cellular Biochemistry
Volume79
Issue number1
Publication statusPublished - 2000
Publication categoryResearch
Peer-reviewedYes