Extracellular Electron Transfer by the Gram-positive Bacterium Enterococcus faecalis

Research output: Contribution to journalArticle

Abstract

Extracellular electron transfer (EET) in microbial cells is essential for certain biotechnological applications and contributes to the biogeochemical cycling of elements and syntrophic microbial metabolism in complex natural environments. The Gram-positive lactic acid bacterium Enterococcus faecalis, an opportunistic human pathogen, is shown to be able to transfer electrons generated in fermentation metabolism to electrodes directly and indirectly via mediators. By exploiting E. faecalis wild-type and mutant cells it is demonstrated that reduced demethylmenaquinone in the respiratory chain in the bacterial cytoplasmic membrane is crucial for the EET. Heme-proteins are not involved and cytochrome bd oxidase activity was found to attenuate EET. These results are significant for the mechanistic understanding of EET in bacteria and for design of microbial electrochemical systems. The basic findings infer that in dense microbial communities, such as in biofilm and in the large intestine, metabolism in E. faecalis and similar Gram-positive lactic acid bacteria might be electrically connected to other microbes. Such an intercellular electron transfer might confer syntrophic metabolism that promote growth and other activities of bacteria in the microbiota of humans and animals.

Details

Authors
Organisations
External organisations
  • National University of Ireland Galway
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology
  • Cell Biology
  • Biochemistry and Molecular Biology
Original languageEnglish
Pages (from-to)4597-4603
JournalBiochemistry
Volume57
Publication statusPublished - 2018
Publication categoryResearch
Peer-reviewedYes

Related research output

Galina Pankratova, 2018 Oct, Lund: Media-Tryck, Lund University, Sweden. 144 p.

Research output: ThesisDoctoral Thesis (compilation)

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