Extracellular Electron Transfer by the Gram-positive Bacterium Enterococcus faecalis

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Extracellular Electron Transfer by the Gram-positive Bacterium Enterococcus faecalis. / Pankratova, Galina; Leech, Dónal; Gorton, Lo; Hederstedt, Lars.

In: Biochemistry, Vol. 57, 2018, p. 4597-4603.

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TY - JOUR

T1 - Extracellular Electron Transfer by the Gram-positive Bacterium Enterococcus faecalis

AU - Pankratova, Galina

AU - Leech, Dónal

AU - Gorton, Lo

AU - Hederstedt, Lars

PY - 2018

Y1 - 2018

N2 - Extracellular electron transfer (EET) in microbial cells is essential for certain biotechnological applications and contributes to the biogeochemical cycling of elements and syntrophic microbial metabolism in complex natural environments. The Gram-positive lactic acid bacterium Enterococcus faecalis, an opportunistic human pathogen, is shown to be able to transfer electrons generated in fermentation metabolism to electrodes directly and indirectly via mediators. By exploiting E. faecalis wild-type and mutant cells it is demonstrated that reduced demethylmenaquinone in the respiratory chain in the bacterial cytoplasmic membrane is crucial for the EET. Heme-proteins are not involved and cytochrome bd oxidase activity was found to attenuate EET. These results are significant for the mechanistic understanding of EET in bacteria and for design of microbial electrochemical systems. The basic findings infer that in dense microbial communities, such as in biofilm and in the large intestine, metabolism in E. faecalis and similar Gram-positive lactic acid bacteria might be electrically connected to other microbes. Such an intercellular electron transfer might confer syntrophic metabolism that promote growth and other activities of bacteria in the microbiota of humans and animals.

AB - Extracellular electron transfer (EET) in microbial cells is essential for certain biotechnological applications and contributes to the biogeochemical cycling of elements and syntrophic microbial metabolism in complex natural environments. The Gram-positive lactic acid bacterium Enterococcus faecalis, an opportunistic human pathogen, is shown to be able to transfer electrons generated in fermentation metabolism to electrodes directly and indirectly via mediators. By exploiting E. faecalis wild-type and mutant cells it is demonstrated that reduced demethylmenaquinone in the respiratory chain in the bacterial cytoplasmic membrane is crucial for the EET. Heme-proteins are not involved and cytochrome bd oxidase activity was found to attenuate EET. These results are significant for the mechanistic understanding of EET in bacteria and for design of microbial electrochemical systems. The basic findings infer that in dense microbial communities, such as in biofilm and in the large intestine, metabolism in E. faecalis and similar Gram-positive lactic acid bacteria might be electrically connected to other microbes. Such an intercellular electron transfer might confer syntrophic metabolism that promote growth and other activities of bacteria in the microbiota of humans and animals.

U2 - 10.1021/acs.biochem.8b00600

DO - 10.1021/acs.biochem.8b00600

M3 - Article

VL - 57

SP - 4597

EP - 4603

JO - Biochemistry

T2 - Biochemistry

JF - Biochemistry

SN - 0006-2960

ER -