Extrachromosomal RNA-DNA complex containing long telomeric repeats in chironomids.

Research output: Contribution to journalArticle

Abstract

We have analysed an extracted RNase sensitive fraction containing telomeric repeat sequences in the telomerase negative dipteran Chironomus tentans. It shows a slow and well-defined electophoretic migration corresponding to > 20 kb and is sensitive not only to RNase, but also to DNase. It hybridizes to both strands of the telomeric repeat with about equal intensities. DNA is probably the dominant component since the fraction is only slightly heavier than genomic DNA in isopycnic gradients but considerably lighter than RNA. It can, nevertheless, be shown to incorporate tritiated uridine. The material might represent another example of extrachromosomal telomeric repeats in telomerase negative cells.

Details

Authors
  • Monika Rosén
  • I Kamnert
  • J-E Edström
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Genetics

Keywords

  • Animal, Northern, Non-U.S. Gov't, Support, Nucleic Acid, Repetitive Sequences, RNA, Genes, Insect, DNA Probes, DNA, Chironomidae : genetics, Blotting, Centrifugation, Isopycnic
Original languageEnglish
Pages (from-to)167-174
JournalInsect Molecular Biology
Volume11
Issue number2
Publication statusPublished - 2002
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Genetics (Closed 2011) (011005100), Invertebral Developmental Biology, Udo Haecker's group (013212048)

Related research output

Rosén, M., 2001, Department of Genetics, Lund University. 130 p.

Research output: ThesisDoctoral Thesis (compilation)

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