Extraction with Water-in-Carbon Dioxide Microemulsions: A Case Study on Steviol Glycosides

Research output: Contribution to journalArticle


This work explores the use of water-in-supercritical carbon dioxide (scCO2) microemulsions for the extraction of polar metabolites from plants. Stevia rebaudiana Bertoni leaves and polyethylene glycol trimethylnonyl ether (TMN) surfactants were selected for a case study. A CO2-water-TMN 10 mixture at 35 °C and 30.0 MPa extracted 7 mg target analyte/g dry leaves. The extraction was proven to occur due to a water-surfactant liquid solution rather than a water-in-CO2 microemulsion. Using a modified extraction setup, the microemulsion was created prior to extraction. TMN 6 was able to dissolve enough water in CO2 to extract steviol glycosides, in detectable but very small amounts.


Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Analytical Chemistry


  • Bertoni leaves, CO supercritical extraction, Green solvent, Microemulsions, Polyethylene glycol trimethylnonyl ether
Original languageEnglish
Number of pages10
JournalJournal of Surfactants and Detergents
Publication statusE-pub ahead of print - 2019 Jul 5
Publication categoryResearch