Factors influencing the skin exposure to diphenylguanidine in surgical gloves

Research output: Contribution to journalPublished meeting abstract


Background: For health care personnelhandhygieneandwearing appropriate protective gloves are compulsory. We have noticed an increase of hand eczema caused by contact allergy from rubber chemicals such as diphenylguanidine (DPG) in synthetic rubber gloves used by surgeons and surgical nurses. The exposure time to surgical gloves as well as the number of gloves used per day vary. Furthermore surgery very often includes the use of skin disinfectants before glove donning. Objectives: Does the exposure time influence the amount of DPG released from the gloves? Does the exposure of skin disinfectants before glove donning influence the release of DPG? Methods: Using synthetic sweat, the amount of DPG released from the inside of the gloves at different timeswas measured by HPLC. The remaining extractable DPG was estimated by washing the inside of the gloves with ethanol (50% in water) for 10min. The amount of DPG found on the hands exposed to 3ml skin disinfectant allowed to dry prior to glove donning was compared to the amount found on unexposed hands. After wearing gloves for 60 min the handswerewashedwith ethanol and the amount of DPG recovered was analysed with HPLC. Results: After 10, 30, 60 and 180 min approximately 73%, 79%, 82%, 87% of the total amount of DPG was released. For all comparisons the amount of DPG released was higher on the hand exposed to disinfectants compared with the unexposed hand. Conclusions: A comparatively short exposure time to gloves (10min) implies a substantial exposure to DPG. Thus, repeated usage of glovesmight enhance skin exposure to this rubber chemical. Skin disinfectants does increase the amount of DPG released from the gloves. Thus, possibly the components of the disinfectant remaining on the skin or some othermechanism add to the exposure to DPG.


External organisations
  • Skåne University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Environmental Health and Occupational Health


  • Gloves, Contact allergic dermatitis, disinfectant agent, rubber, alcohol, water, glove, skin allergy, skin, exposure, surgical glove, society, contact dermatitis, human, protective clothing, surgery, nurse, surgeon, health care, high performance liquid chromatography, sweat, contact allergy, hand eczema
Original languageEnglish
Article number194
Pages (from-to)59-60
JournalContact Dermatitis
Issue numberSuppl s1
Publication statusPublished - 2014 Jun 1
Publication categoryResearch
Event12th Contress of the European Society of Contact Dermatitis (ESCD) - Barcelona, Spain
Duration: 2014 Jun 252014 Jun 28