Fast egg collection method greatly improves randomness of egg sampling in Drosophila melanogaster

Research output: Contribution to journalArticle

Abstract

When obtaining samples for population genetic studies, it is essential that the sampling is random. For Drosophila, one of the crucial steps in sampling experimental flies is the collection of eggs. Here an egg collection method is presented, which randomizes the eggs in a water column and diminishes environmental variance. This method was compared with a traditional egg collection method where eggs are collected directly from the medium. Within each method the observed and expected standard deviations of egg-to-adult viability were compared, whereby the difference in the randomness of the samples between the two methods was assessed. The method presented here was superior to the traditional method. Only 14% of the samples had a standard deviation higher than expected, as compared with 58% in the traditional method. To reduce bias in the estimation of the variance and the mean of a trait and to obtain a representative collection of genotypes, the method presented here is strongly recommended when collecting eggs from Drosophila.

Details

Authors
External organisations
  • Aarhus University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Ecology
  • Genetics

Keywords

  • Egg-to-adult viability, density control, random sampling, reliability, sampling error
Original languageEnglish
Pages (from-to)44-46
Number of pages3
JournalFly
Volume7
Issue number1
Publication statusPublished - 2013
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes