Fasting reveals largely intact systemic lipid mobilization mechanisms in respiratory chain complex III deficient mice

Research output: Contribution to journalArticle

Abstract

Mice homozygous for the human GRACILE syndrome mutation (Bcs1lc.A232G) display decreased respiratory chain complex III activity, liver dysfunction, hypoglycemia, rapid loss of white adipose tissue and early death. To assess the underlying mechanism of the lipodystrophy in homozygous mice (Bcs1lp.S78G), these and wild-type control mice were subjected to a short 4-hour fast. The homozygotes had low baseline blood glucose values, but a similar decrease in response to fasting as in wild-type mice, resulting in hypoglycemia in the majority. Despite the already depleted glycogen and increased triacylglycerol content in the mutant livers, the mice responded to fasting by further depletion and increase, respectively. Increased plasma free fatty acids (FAs) upon fasting suggested normal capacity for mobilization of lipids from white adipose tissue into circulation. Strikingly, however, serum glycerol concentration was not increased concomitantly with free FAs, suggesting its rapid uptake into the liver and utilization for fuel or gluconeogenesis in the mutants. The mutant hepatocyte mitochondria were capable of responding to fasting by appropriate morphological changes, as analyzed by electron microscopy, and by increasing respiration. Mutants showed increased hepatic gene expression of major metabolic controllers typically associated with fasting response (Ppargc1a, Fgf21, Cd36) already in the fed state, suggesting a chronic starvation-like metabolic condition. Despite this, the mutant mice responded largely normally to fasting by increasing hepatic respiration and switching to FA utilization, indicating that the mechanisms driving these adaptations are not compromised by the CIII dysfunction. Summary statement: Bcs1l mutant mice with severe CIII deficiency, energy deprivation and post-weaning lipolysis respond to fasting similarly to wild-type mice, suggesting largely normal systemic lipid mobilization and utilization mechanisms.

Details

Authors
Organisations
External organisations
  • Karolinska University Hospital
  • University of Zagreb
  • Region Skåne
  • Folkhälsan Research Center
  • Mälardalen University
  • University of Helsinki
  • Minerva Foundation Institute for Medical Research
  • Helsinki University Children's Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology

Keywords

  • BCS1L, Fasting, Lipid metabolism, Liver disease, Mitochondrial disorder, OXPHOS
Original languageEnglish
Article number165573
Number of pages11
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1866
Issue number1
Early online date2019 Oct 29
Publication statusPublished - 2020
Publication categoryResearch
Peer-reviewedYes