Flow-injection immunoassays: Present and future

Research output: Contribution to journalArticle

Abstract

The recent development of the flow-injection immunoassay (FIIA), beginning with one of the first publications in 1980 by Lim and Miller, has resulted in a growing field which combines the precise and reproducible timing of flow-injection analysis (FIA) with immunoassays to yield assays which are carried out in a nonequilibrium time frame. These often faster assay methods require small volumes of sample and reduced sample handling. The advancement of this field is briefly reviewed in this paper with respect to the general methods and detection schemes applied. A novel method utilizing sequential injection (SI) is also introduced. The method uses immunomagnetic beads to create a renewable reaction column which does not require in-line regeneration.

Details

Authors
External organisations
  • University of Washington, Seattle
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medicinal Chemistry
Original languageEnglish
Pages (from-to)121-128
Number of pages8
JournalMicrochemical Journal
Volume45
Issue number2
Publication statusPublished - 1992 Jan 1
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes