Fluorescence polarisation for immunoreagent characterisation

Research output: Contribution to journalArticle

Abstract

Antibodies were characterised using fluorescence polarisation, a homogeneous assay technique in which all reagents are in solution. Kinetic studies on the association and dissociation of the immunocomplex were performed. A competitive assay was used and the sensitivities, operational linearities, as well as the specificities of the immunoassays were experimentally determined for various antibody preparations with specificity for triazines. Detection limits for atrazine in water samples were determined to be within the range of 0.08-0.4 ng ml-1 using a 5-min incubation time and a 0.5-ml sample volume.

Details

Authors
Organisations
External organisations
  • Astra Draco AB
Research areas and keywords

Keywords

  • Antibody characterisation, Atrazine, Fluorescence polarisation, Homogenous immunoassay, Triazines
Original languageEnglish
Pages (from-to)31-39
Number of pages9
JournalJournal of Immunological Methods
Volume213
Issue number1
Publication statusPublished - 1998 Apr 1
Publication categoryResearch
Peer-reviewedYes