Focal Adhesion Isolation Assay Using ECM-Coated Magnetic Beads

Research output: Contribution to journalArticle

Abstract

Focal adhesions are force sensitive structures that dynamically alter their composition, protein-protein interactions, and signaling in response to external mechanical stimuli. These dynamic changes are critical for focal adhesion function and are required for cellular mechanosensing. Here, we describe a simple protocol that allows for isolation of the focal adhesion complex from adherent cells in culture in response to different mechanical stimuli applied at adhesion sites. By combining this assay with approaches such as proteomics or western blot analysis, one can study the force-dependent changes in focal adhesion composition, protein-protein interactions and signaling.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology
  • Cell Biology

Keywords

  • Focal adhesion composition, Magnetics, Mechanotransduction
Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalMethods in molecular biology (Clifton, N.J.)
Volume2217
Publication statusPublished - 2021
Publication categoryResearch
Peer-reviewedYes