Formation of Short-Lived Protein Aggregates Directly from the Coil in Two-State Folding

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Formation of Short-Lived Protein Aggregates Directly from the Coil in Two-State Folding. / Silow, Maria; Tan, Yee-Joo; Fersht, Alan R; Oliveberg, Mikael.

In: Biochemistry, Vol. 38, No. 40, 1999, p. 13006-13012.

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Silow, M, Tan, Y-J, Fersht, AR & Oliveberg, M 1999, 'Formation of Short-Lived Protein Aggregates Directly from the Coil in Two-State Folding', Biochemistry, vol. 38, no. 40, pp. 13006-13012. https://doi.org/10.1021/bi9909997

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Silow, Maria ; Tan, Yee-Joo ; Fersht, Alan R ; Oliveberg, Mikael. / Formation of Short-Lived Protein Aggregates Directly from the Coil in Two-State Folding. In: Biochemistry. 1999 ; Vol. 38, No. 40. pp. 13006-13012.

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TY - JOUR

T1 - Formation of Short-Lived Protein Aggregates Directly from the Coil in Two-State Folding

AU - Silow, Maria

AU - Tan, Yee-Joo

AU - Fersht, Alan R

AU - Oliveberg, Mikael

PY - 1999

Y1 - 1999

N2 - Recent results on the 102 residue protein U1A show that protein aggregation is not always slow and irreversible but may take place transiently in refolding studies on a millisecond time scale. In this study we observe a similar aggregation behavior with the classical two-state protein CI2. Since both U1A and CI2 appear to fold directly from the coil at low protein concentrations, it is likely that the aggregates also form directly from the coil. This is in contrast to the behavior of larger multistate proteins where aggregation occurs in connection to "sticky" intermediates.

AB - Recent results on the 102 residue protein U1A show that protein aggregation is not always slow and irreversible but may take place transiently in refolding studies on a millisecond time scale. In this study we observe a similar aggregation behavior with the classical two-state protein CI2. Since both U1A and CI2 appear to fold directly from the coil at low protein concentrations, it is likely that the aggregates also form directly from the coil. This is in contrast to the behavior of larger multistate proteins where aggregation occurs in connection to "sticky" intermediates.

U2 - 10.1021/bi9909997

DO - 10.1021/bi9909997

M3 - Article

VL - 38

SP - 13006

EP - 13012

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 40

ER -