Formation of Supported Lipid Bilayers by Vesicle Fusion: Effect of Deposition Temperature

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Formation of Supported Lipid Bilayers by Vesicle Fusion: Effect of Deposition Temperature. / Lind, Tania Kjellerup; Cardenas, Marite; Wacklin, Hanna.

In: Langmuir, Vol. 30, No. 25, 2014, p. 7259-7263.

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Lind, Tania Kjellerup ; Cardenas, Marite ; Wacklin, Hanna. / Formation of Supported Lipid Bilayers by Vesicle Fusion: Effect of Deposition Temperature. In: Langmuir. 2014 ; Vol. 30, No. 25. pp. 7259-7263.

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TY - JOUR

T1 - Formation of Supported Lipid Bilayers by Vesicle Fusion: Effect of Deposition Temperature

AU - Lind, Tania Kjellerup

AU - Cardenas, Marite

AU - Wacklin, Hanna

PY - 2014

Y1 - 2014

N2 - We have investigated the effect of deposition temperature on supported lipid bilayer formation via vesicle fusion. By using several complementary surface-sensitive techniques, we demonstrate that despite contradicting literature on the subject, high-quality bilayers can be formed below the main phase-transition temperature of the lipid. We have carefully studied the formation mechanism of supported DPPC bilayers below and above the lipid melting temperature (T-m) by quartz crystal microbalance and atomic force microscopy under continuous flow conditions. We also measured the structure of lipid bilayers formed below or above T-m by neutron reflection and investigated the effect of subsequent cooling to below the T-m. Our results clearly show that a continuous supported bilayer can be formed with high surface coverage below the lipid T-m. We also demonstrate that the high dissipation responses observed during the deposition process by QCM-D correspond to vesicles absorbed on top of a continuous bilayer and not to a surface-supported vesicular layer as previously reported.

AB - We have investigated the effect of deposition temperature on supported lipid bilayer formation via vesicle fusion. By using several complementary surface-sensitive techniques, we demonstrate that despite contradicting literature on the subject, high-quality bilayers can be formed below the main phase-transition temperature of the lipid. We have carefully studied the formation mechanism of supported DPPC bilayers below and above the lipid melting temperature (T-m) by quartz crystal microbalance and atomic force microscopy under continuous flow conditions. We also measured the structure of lipid bilayers formed below or above T-m by neutron reflection and investigated the effect of subsequent cooling to below the T-m. Our results clearly show that a continuous supported bilayer can be formed with high surface coverage below the lipid T-m. We also demonstrate that the high dissipation responses observed during the deposition process by QCM-D correspond to vesicles absorbed on top of a continuous bilayer and not to a surface-supported vesicular layer as previously reported.

U2 - 10.1021/la500897x

DO - 10.1021/la500897x

M3 - Article

VL - 30

SP - 7259

EP - 7263

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 25

ER -