Formation of the alpha 1-microglobulin chromophore in mammalian and insect cells: a novel post-translational mechanism?

Research output: Contribution to journalArticle

Abstract

alpha 1-Microglobulin is an immunosuppressive plasma protein synthesized by the liver. The isolated protein is yellow-brown, but the hypothetical chromophore has not yet been identified. In this work, it is shown that a human liver cell line, HepG2, grown in a completely synthetic and serum-free medium, secretes alpha 1-microglobulin which is also yellow-brown, suggesting a de novo synthesis of the chromophore by the cells. alpha 1-Microglobulin isolated from the culture medium of insect cells transfected with the gene for rat alpha 1-microglobulin is also yellow-brown, suggesting that the gene carries information about the chromophore. Reduction and alkylation or removal of N- or O-linked carbohydrates by glycosidase treatment did not reduce the colour intensity of the protein. An internal dodecapeptide (amino acid positions 70-81 in human alpha 1-microglobulin) was also yellow-brown. The latter results indicate that the chromophore is linked to the polypeptide. In conclusion, the results suggest that the alpha 1-microglobulin gene carries information activating a post-translational protein modification mechanism which is present in mammalian and insect cells.

Details

Authors
Organisations
External organisations
  • Lund University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area

Keywords

  • Alkylation, Alpha-Globulins/chemistry, Amino Acid Sequence, Animals, Cell Line, Glycosylation, Humans, Molecular Sequence Data, Moths, Oxidation-Reduction, Peptides/chemistry, Pigments, Biological/chemistry, Protein Processing, Post-Translational, Tumor Cells, Cultured
Original languageEnglish
Pages (from-to)50-4
JournalFEBS Letters
Volume362
Issue number1
Publication statusPublished - 1995 Mar 27
Publication categoryResearch
Peer-reviewedYes