Functional analysis of the mouse J chain

Research output: ThesisDoctoral Thesis (compilation)

Standard

Functional analysis of the mouse J chain. / Erlandsson, Lena.

Immunology Unit, Sölvegatan 21, 223 62 Lund, Sweden, 1999. 140 p.

Research output: ThesisDoctoral Thesis (compilation)

Harvard

Erlandsson, L 1999, 'Functional analysis of the mouse J chain', Doctor, Immunology.

APA

Erlandsson, L. (1999). Functional analysis of the mouse J chain. Immunology Unit, Sölvegatan 21, 223 62 Lund, Sweden.

CBE

Erlandsson L. 1999. Functional analysis of the mouse J chain. Immunology Unit, Sölvegatan 21, 223 62 Lund, Sweden. 140 p.

MLA

Erlandsson, Lena Functional analysis of the mouse J chain Immunology Unit, Sölvegatan 21, 223 62 Lund, Sweden. 1999.

Vancouver

Erlandsson L. Functional analysis of the mouse J chain. Immunology Unit, Sölvegatan 21, 223 62 Lund, Sweden, 1999. 140 p.

Author

Erlandsson, Lena. / Functional analysis of the mouse J chain. Immunology Unit, Sölvegatan 21, 223 62 Lund, Sweden, 1999. 140 p.

RIS

TY - THES

T1 - Functional analysis of the mouse J chain

AU - Erlandsson, Lena

N1 - Defence details Date: 1999-05-05 Time: 10:15 Place: Sölvegatan 21, Lund External reviewer(s) Name: Sideras, Paschalis Title: Dr Affiliation: Umeå University, Sweden ---

PY - 1999

Y1 - 1999

N2 - We have in this study analyzed the function of the murine Joining chain (J chain) in vivo. J chain is a polypeptide found in sera and secretions in complex with pentameric IgM and dimeric IgA. J chain is expressed in terminally differentiated B lymphocytes and needed for the transport of dimeric IgA and pentameric IgM over mucosal surfaces via the poly Ig receptor. A mouse deficient for J chain was generated and it was shown that J chain in vivo seems to be necessary for efficient assembly and secretion of polymeric IgM. Secondly, in the absence of J chain, antigen-specific IgA immunity in the lamina propria of the mucosa cannot mediate protection on the mucosal surface of the intestinal lumen; thereby demonstrating in vivo a direct relationship between mucosal transport of secretory IgA and intestinal immune protection. A second mouse was generated where the Diphtheria toxin A gene was introduced into the J chain locus, thereby ablating J chain expressing cells. Evidence was found for the existence of two separate plasma cell populations distinguishable by the absence or the presence of J chain. The dogma that in mice the J chain is expressed in all plasma cells irrespective of isotype has been challenged.

AB - We have in this study analyzed the function of the murine Joining chain (J chain) in vivo. J chain is a polypeptide found in sera and secretions in complex with pentameric IgM and dimeric IgA. J chain is expressed in terminally differentiated B lymphocytes and needed for the transport of dimeric IgA and pentameric IgM over mucosal surfaces via the poly Ig receptor. A mouse deficient for J chain was generated and it was shown that J chain in vivo seems to be necessary for efficient assembly and secretion of polymeric IgM. Secondly, in the absence of J chain, antigen-specific IgA immunity in the lamina propria of the mucosa cannot mediate protection on the mucosal surface of the intestinal lumen; thereby demonstrating in vivo a direct relationship between mucosal transport of secretory IgA and intestinal immune protection. A second mouse was generated where the Diphtheria toxin A gene was introduced into the J chain locus, thereby ablating J chain expressing cells. Evidence was found for the existence of two separate plasma cell populations distinguishable by the absence or the presence of J chain. The dogma that in mice the J chain is expressed in all plasma cells irrespective of isotype has been challenged.

KW - Immunology

KW - diphtheria toxin A

KW - plasma cell

KW - mucosa

KW - J chain

KW - knock-out

KW - serology

KW - transplantation

KW - Immunologi

KW - serologi

M3 - Doctoral Thesis (compilation)

SN - 91-628-3544-0

PB - Immunology Unit, Sölvegatan 21, 223 62 Lund, Sweden

ER -