Functional state of the β cell affects expression of both forms of glutamic acid decarboxylase

Research output: Contribution to journalArticle

Abstract

Glutamic acid decarboxylase (GAD) is a candidate target autoantigen involved in the pathogenesis of insulin-dependent diabetes mellitus (IDDM). The functional state of the B cells has been suggested to play a pathogenic role in IDDM by altering β-cell autoantigen expression. In this study, we investigated expression of GAD-65 and GAD-67 in isolated Sprague-Dawley rat islets cultured at different glucose concentrations. Using GAD isoform-specific antibodies in an immunoblot assay, we found that expression of both GAD-65 and GAD-67 in cultured islets was glucose dependent and that increased expression of both forms of GAD correlated with increased functional state of the β cell. Our data indicate that the functional state of the β cell influences islet cell expression of GAD. Thus, decreasing islet cell expression of GAD by suppressing β cells activity may have a potential role in blunting the autoimmune destruction of pancreatic islet B cells.

Details

Authors
External organisations
  • University of Washington
Research areas and keywords

Keywords

  • Glutamic acid decarboxylase, Insulin-dependent diabetes mellitus, β cell function
Original languageEnglish
Pages (from-to)558-562
JournalPancreas
Volume9
Issue number5
Publication statusPublished - 1994 Jan 1
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes