Functional state of the β cell affects expression of both forms of glutamic acid decarboxylase
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Glutamic acid decarboxylase (GAD) is a candidate target autoantigen involved in the pathogenesis of insulin-dependent diabetes mellitus (IDDM). The functional state of the B cells has been suggested to play a pathogenic role in IDDM by altering β-cell autoantigen expression. In this study, we investigated expression of GAD-65 and GAD-67 in isolated Sprague-Dawley rat islets cultured at different glucose concentrations. Using GAD isoform-specific antibodies in an immunoblot assay, we found that expression of both GAD-65 and GAD-67 in cultured islets was glucose dependent and that increased expression of both forms of GAD correlated with increased functional state of the β cell. Our data indicate that the functional state of the β cell influences islet cell expression of GAD. Thus, decreasing islet cell expression of GAD by suppressing β cells activity may have a potential role in blunting the autoimmune destruction of pancreatic islet B cells.
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|Publication status||Published - 1994 Jan 1|