Functionally involved cell surface antigens on murine lymphokine activated killer cells

Research output: Contribution to journalArticle

Abstract

To investigate the possible involvement of some cell surface structures on lymphoid cells in the functional activity of lymphokine activated killer (LAK) cells, a number of monoclonal antibodies (Mab) against such structures was studied for their ability to inhibit LAK activity in a standard cytotoxicity assay against the natural killer-insensitive target cell EL-4. Almost complete inhibition of LAK activity resulted from incubation with antibodies to the LFA-1 antigen, while blocking of the Lyt 2 antigen reduced cytotoxic activity about 50%. Mab to T-200 gave a weak and inconsistent inhibitory activity, while antibodies to Thy 1, L3T4, IL-2 receptor and MHC class I antigens were without effect. Mab to LFA-1 and Lyt 2 inhibited LAK activity towards EL-4, YAC-1 and differentiated F-9 teratocarcinoma cells, but did not affect LAK-mediated killing of undifferentiated F-9 cells. Experiments with separate preincubation of effector and target cells revealed that both LFA-1 and Lyt 2 inhibited LAK activity at the effector cell level only.

Details

Authors
  • Hans Belfrage
  • Pallonji Bhiladvala
  • T Kalland
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Basic Medicine
Original languageEnglish
Pages (from-to)293-8
JournalImmunology Letters
Volume14
Issue number4
Publication statusPublished - 1987
Publication categoryResearch
Peer-reviewedYes

Related research output

Belfrage, H., 1996, Department of Cell and Molecular Biology, Lund University. 58 p.

Research output: ThesisDoctoral Thesis (compilation)

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