Galectin-3 guides intracellular trafficking of some human serotransferrin glycoforms

Research output: Contribution to journalArticle


title = "Galectin-3 guides intracellular trafficking of some human serotransferrin glycoforms",
abstract = "Transferrin internalization via clathrin-mediated endocytosis and subsequent recycling after iron delivery has been extensively studied. Here we demonstrate a previously unrecognized parameter regulating this recycling -the binding of galectin-3 to particular glycoforms of transferrin. Two fractions of transferrin, separated by affinity chromatography based on their binding or not to galectin-3, are targeted to kinetically different endocytic pathways in HFL-1 cells expressing galectin-3 but not in SKBR3 cells lacking galectin-3; the SKBR3 cells, however can acquire the ability to target these transferrin glycoforms differently after preloading with exogenously added galectin-3. In all, this study provides the first evidence of a functional role for transferrin glycans, in intracellular trafficking after uptake. Moreover, the galectin-3 bound glycoform increased in cancer, suggesting a pathophysiological regulation. These are novel aspects of transferrin cell biology, which has previously considered only degree of iron loading, but not other forms of heterogeneity.",
keywords = "Galectin, transferrin, glycoforms, trafficking, endocytosis",
author = "Michael Carlsson and Per Bengtson and Helena Cucak and Hakon Leffler",
year = "2013",
doi = "10.1074/jbc.M113.487793",
language = "English",
volume = "288",
pages = "28398--28408",
journal = "Journal of Biological Chemistry",
issn = "1083-351X",
publisher = "ASBMB",
number = "39",