Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury
Research output: Contribution to journal › Article
Abstract
Transfusion-related acute lung injury (TRALI) is a syndrome of respiratory distress upon blood transfusion and is the leading cause of transfusion-related fatalities. Whether the gut microbiota plays any role in the development of TRALI is currently unknown. We observed that untreated barrier-free (BF) mice suffered from severe antibody-mediated acute lung injury, whereas the more sterile housed specific pathogen-free (SPF) mice and gut flora-depleted BF mice were both protected from lung injury. The prevention of TRALI in the SPF mice and gut flora-depleted BF mice was associated with decreased plasma macrophage inflammatory protein-2 levels as well as decreased pulmonary neutrophil accumulation. DNA sequencing of amplicons of the 16S ribosomal RNA gene revealed a varying gastrointestinal bacterial composition between BF and SPF mice. BF fecal matter transferred into SPF mice significantly restored TRALI susceptibility in SPF mice. These data reveal a link between the gut flora composition and the development of antibody-mediated TRALI in mice. Assessment of gut microbial composition may help in TRALI risk assessment before transfusion.
Details
Authors | |
---|---|
Organisations | |
External organisations |
|
Research areas and keywords | Subject classification (UKÄ) – MANDATORY
|
Original language | English |
---|---|
Pages (from-to) | 1651-1663 |
Journal | Blood Advances |
Volume | 2 |
Issue number | 13 |
Publication status | Published - 2018 Jul 10 |
Publication category | Research |
Peer-reviewed | Yes |