Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury

Research output: Contribution to journalArticle


title = "Gastrointestinal microbiota contributes to the development of murine transfusion-related acute lung injury",
abstract = "Transfusion-related acute lung injury (TRALI) is a syndrome of respiratory distress upon blood transfusion and is the leading cause of transfusion-related fatalities. Whether the gut microbiota plays any role in the development of TRALI is currently unknown. We observed that untreated barrier-free (BF) mice suffered from severe antibody-mediated acute lung injury, whereas the more sterile housed specific pathogen-free (SPF) mice and gut flora-depleted BF mice were both protected from lung injury. The prevention of TRALI in the SPF mice and gut flora-depleted BF mice was associated with decreased plasma macrophage inflammatory protein-2 levels as well as decreased pulmonary neutrophil accumulation. DNA sequencing of amplicons of the 16S ribosomal RNA gene revealed a varying gastrointestinal bacterial composition between BF and SPF mice. BF fecal matter transferred into SPF mice significantly restored TRALI susceptibility in SPF mice. These data reveal a link between the gut flora composition and the development of antibody-mediated TRALI in mice. Assessment of gut microbial composition may help in TRALI risk assessment before transfusion.",
author = "Rick Kapur and Michael Kim and Johan Rebetz and Bj{\"o}rn Hallstr{\"o}m and Bj{\"o}rkman, {Jonas T} and Alisa Takabe-French and Noel Kim and Jonathan Liu and Shanjeevan Shanmugabhavananthan and Stefan Milosevic and McVey, {Mark J} and Speck, {Edwin R} and Semple, {John W}",
note = "{\circledC} 2018 by The American Society of Hematology.",
year = "2018",
month = "7",
day = "10",
doi = "10.1182/bloodadvances.2018018903",
language = "English",
volume = "2",
pages = "1651--1663",
journal = "Blood Advances",
issn = "2473-9529",
publisher = "American Society of Hematology",
number = "13",