Genome-wide meta-analysis identifies BARX1 and EML4-MTA3 as new loci associated with infantile hypertrophic pyloric stenosis

Research output: Contribution to journalArticle

Abstract

Infantile hypertrophic pyloric stenosis (IHPS) is a disorder of young infants with a population incidence of ∼2/1000 live births, caused by hypertrophy of the pyloric sphincter smooth muscle. Reported genetic loci associated with IHPS explain only a minor proportion of IHPS risk. To identify new risk loci, we carried out a genome-wide meta-analysis on 1395 surgery-confirmed cases and 4438 controls, with replication in a set of 2427 cases and 2524 controls. We identified and replicated six independent genomic loci associated with IHPS risk at genome wide significance (P < 5 × 10-8), including novel associations with two single nucleotide polymorphisms (SNPs). One of these SNPs, rs6736913 [odds ratio (OR) = 2.32; P = 3.0 × 10-15], is a low frequency missense variant in EML4 at 2p21. The second SNP, rs1933683 (OR = 1.34; P = 3.1 × 10-9) is 1 kb downstream of BARX1 at 9q22.32, an essential gene for stomach formation in embryogenesis. Using the genome-wide complex trait analysis method, we estimated the IHPS SNP heritability to be 30%, and using the linkage disequilibrium score regression method, we found support for a previously reported genetic correlation of IHPS with lipid metabolism. By combining the largest collection of IHPS cases to date (3822 cases), with results generalized across populations of different ancestry, we elucidate novel mechanistic avenues of IHPS disease architecture.

Details

Authors
  • João Fadista
  • Line Skotte
  • Frank Geller
  • Jonas Bybjerg-Grauholm
  • Sanne Gørtz
  • Paul A. Romitti
  • Michele Caggana
  • Denise M. Kay
  • Hans Matsson
  • Heather A. Boyd
  • David M. Hougaard
  • Agneta Nordenskjöld
  • James L. Mills
  • Mads Melbye
  • Bjarke Feenstra
Organisations
External organisations
  • Danish Serum Institute, Copenhagen
  • University of Iowa
  • Wadsworth Center for Laboratories and Research
  • Karolinska Institutet
  • Karolinska University Hospital
  • National Institute of Child Health and Human Development
  • University of Copenhagen
  • Stanford University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medical Genetics
  • Pediatrics
Original languageEnglish
Pages (from-to)332-340
Number of pages9
JournalHuman Molecular Genetics
Volume28
Issue number2
Publication statusPublished - 2019
Publication categoryResearch
Peer-reviewedYes