Glioblastoma multiforme in the microscope: Diagnostic features of importance for prognosis and treatment.

Research output: ThesisDoctoral Thesis (compilation)

Abstract

Glioblastoma multiforme (GBM) is the most malignant and also the most common form of brain tumour in humans. The overall aim of this thesis was to investigate morphological and diagnostics features in human GBM that may be of importance for prognosis and treatment.

Immunohistochemically stained tissue sections have been microscopically analysed regarding immuno-reactive cells, cell proliferation, telomerase activity and presence of coxsackie adenovirus receptor (CAR). While telomerase activity reflects inherent life prolongation, CAR act as adhesion molecules on target cells in adenoviral based gene therapy.

We found that cytotoxic T-lymfocytes (CTL) occurred spontaneously in GBM but also that a transient increase was detected after immunotherapy. Macrophage positive cells were in general plentiful.

GBM as well as other grades of gliomas, expressed low levels of CAR while neuroblastomas and medulloblastomas exhibited a high CAR expression. However, various regions and cell types of the normal brain also expressed CAR.

Proliferation activity (Ki-67) showed a significant correlation to longer survival if index was below 10 %, while telomerase activity (hTERT) showed no relation to length of survival. Different methods of assessment of Ki-67 are of relevance for histopathological diagnostics.

This project indicates that morphological features of importance for prognosis and treatment can be identified by the use of established and simple, reliable methods, routinely applied in clinical pathological diagnostics.

Details

Authors
  • Annette Persson
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology

Keywords

  • General pathology, cytogenetik, Genetik, Genetics, cytogenetics, Medicin (människa och djur), macrophages, adenoviral based gene-therapy proliferation activity, telomerase activity, immunohistochemistry, Medicine (human and vertebrates), cytotoxic T-lymphocytes, diagnostic features, prognosis, immuno-therapy, Glioblastoma multiforme, neuropathology, pathological anatomy, Patologi (allmän), patologisk anatomi
Original languageEnglish
QualificationDoctor
Awarding Institution
Supervisors/Assistant supervisor
Award date2007 May 16
Publisher
  • Annette Persson Department of Pathology, Lund University
Print ISBNs978-91-85559-62-6
Publication statusPublished - 2007
Publication categoryResearch

Bibliographic note

Defence details Date: 2007-05-16 Time: 13:00 Place: the Lecture Hall Department of Pathology Lund University Hospital External reviewer(s) Name: Nordborg, Claes Title: Associate professor Affiliation: Göteborg --- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000)