Glioma cell activation by Alzheimer's peptide Abeta1-42, alpha1-antichymotrypsin, and their mixture.

Research output: Contribution to journalArticle


title = "Glioma cell activation by Alzheimer's peptide Abeta1-42, alpha1-antichymotrypsin, and their mixture.",
abstract = "We compared the effects of Alzheimer’s peptide (Ab1–42), a1-antichymotrypsin (ACT) and an ACT/Ab1–42 mixture on human glioma DK-MG cells. The solution of Ab (5 mM) formed by 2-h incubation at room temperature induced tumour necrosis factor-a (TNF-a) and interleukin (IL)-6 levels by 55 and 45{\%}, respectively, and increased gelatinase B activity by 67{\%}, while exposure of cells to the ACT/Ab1–42 mixture (1:10 molar ratio ACT: Ab1–42) under the same experimental conditions showed no effect on IL-6 levels or gelatinase B activity, but strongly induced TNF-a (by 190{\%}), compared to the con- CMLS, Cell. Mol. Life Sci. 59 (2002) 1734–1743 1420-682X/02/101734-11 {\circledC} Birkh{\"a}user Verlag, Basel, 2002 CMLS Cellular and Molecular Life Sciences trols. Stimulation of the cells with Ab1–42 alone, but not with ACT, increased by about 20{\%} low-density lipoprotein (LDL) uptake and mRNA levels for LDL receptor and HMG-CoA reductase, while the ACT/Ab1–42 mixture significantly increased LDL uptake (by 50{\%}), up-regulated mRNA levels for LDL receptor and HMG-CoA reductase by 48 and 63{\%}, respectively, and increased lipid accumulation by about 20-fold. These data suggest a possible new role for Ab in Alzheimer’s disease through its interaction with the inflammatory reactant, ACT.",
keywords = "Kinetics, Peptide Fragments: chemical synthesis, Human, Interleukin-6: genetics, DNA Primers, DNA, Neoplastic: drug effects, Glioma: physiopathology, Amyloid beta-Protein: pharmacology, Base Sequence, Amyloid beta-Protein: chemical synthesis, Gene Expression Regulation, Neoplasm: biosynthesis, Gelatinase B: metabolism, Peptide Fragments: pharmacology, RNA, Messenger: genetics, Reverse Transcriptase Polymerase Chain Reaction, Support, Non-U.S. Gov't, Thymidine: metabolism, Transcription, Genetic: drug effects, Tumor Cells, Cultured, Tumor Necrosis Factor: genetics, alpha 1-Antichymotrypsin: pharmacology",
author = "Yongxin Sun and Wright, {H T} and Sabina Janciauskiene",
year = "2002",
doi = "10.1007/PL00012501",
language = "English",
volume = "59",
pages = "1734--43",
journal = "Cellular and Molecular Life Sciences",
issn = "1420-9071",
publisher = "Birkha{\"u}ser",
number = "10",