Glucose and xylose co-fermentation of pretreated wheat straw using mutants of S. cerevisiae TMB3400.
Research output: Contribution to journal › Article
Wheat straw was pretreated and fermented to ethanol. Two strains, which had been mutated from the genetically modified Saccharomyces cerevisiae TMB3400, KE6-12 and KE6-13i, have been used in this study and the results of performance were compared to that of the original strain. The glucose and xylose co-fermentation ability was investigated in batch fermentation of steam-pretreated wheat straw (SPWS) liquid (undiluted, and diluted 1.5 and 2 times). Both strains showed improved xylose uptake in diluted SPWS liquid, and increased ethanol yields compared with the original TMB3400 strain, although xylitol formation also increased slightly. In undiluted SPWS liquid, however, only KE6-13i performed better than the original strain regarding xylose utilization. Fed-batch fermentation of 1.5 and 2 times diluted liquid was performed by adding the glucose-rich hydrolysates from enzymatic hydrolysis of the solid fraction of SPWS at a constant feed rate after 5h of fermentation, when the glucose had been depleted. The modified strains showed improved xylose conversion; however, the ethanol yield was not significantly improved due to increased glycerol production. Fed-batch fermentation resulted in faster xylose utilization than in the batch cases.
|Research areas and keywords||
Subject classification (UKÄ) – MANDATORY
|Journal||Journal of Biotechnology|
|Publication status||Published - 2013|
Related research output
Development of integrated cellulose- and starch-based ethanol production and process design for improved xylose conversionErdei, B., 2013, Department of Chemical Engineering, Lund University. 104 p.
Research output: Thesis › Doctoral Thesis (compilation)