Glucose stimulates the biosynthesis of a human pancreatic islet cell protein detected by an antiserum against the human erythrocyte glucose transporter
Research output: Contribution to journal › Article
An antiserum raised against the purified human erythrocyte glucose transporter specifically immunoprecipitated a major protein of Mr36 000 and a minor protein of Mr 58 000 from human pancreatic islets. The biosynthetic incorporation of [35S]methionine into the 36-kDa protein increased 2-3 fold in 16 mM D-glucose with 0.1 mM IBMX as compared to islets incubated in 3 mM D-glucose either with or without IBMX. D-Glucose tested without or with IBMX had no effect on the biosynthesis of the 58-kDa protein. The 36-kDa protein was also detected in rat islets and rat islet tumor cells. It is suggested that this protein may represent a D-glucose and cAMP sensitive component of a glucose transport or glucose binding protein in the pancreatic β-cell.
|Research areas and keywords||
|Number of pages||5|
|Publication status||Published - 1983 Jul 4|