Glucose stimulates the biosynthesis of a human pancreatic islet cell protein detected by an antiserum against the human erythrocyte glucose transporter

Research output: Contribution to journalArticle

Abstract

An antiserum raised against the purified human erythrocyte glucose transporter specifically immunoprecipitated a major protein of Mr36 000 and a minor protein of Mr 58 000 from human pancreatic islets. The biosynthetic incorporation of [35S]methionine into the 36-kDa protein increased 2-3 fold in 16 mM D-glucose with 0.1 mM IBMX as compared to islets incubated in 3 mM D-glucose either with or without IBMX. D-Glucose tested without or with IBMX had no effect on the biosynthesis of the 58-kDa protein. The 36-kDa protein was also detected in rat islets and rat islet tumor cells. It is suggested that this protein may represent a D-glucose and cAMP sensitive component of a glucose transport or glucose binding protein in the pancreatic β-cell.

Details

Authors
External organisations
  • Hagedorn Research Institute
Research areas and keywords

Keywords

  • 3-Isobutyl-1-methylxanthine, Glucose regulation, Glucose transport, Human erythrocyte glucose transporter, Human pancreatic islet, β-Cell protein
Original languageEnglish
Pages (from-to)331-335
Number of pages5
JournalFEBS Letters
Volume157
Issue number2
Publication statusPublished - 1983 Jul 4
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes