Glycoside hydrolases for extraction and modifi cation of polyphenolic antioxidants

Research output: Chapter in Book/Report/Conference proceedingBook chapter

Abstract

Antioxidants are important molecules that are widely used by humans, both as dietary supplements and as additives to different types of products. In this chapter, we review how flavonoids, a class of polyphenolic antioxidants that are often found in glycosylated forms in many natural resources, can be extracted and modifi ed using glycoside hydrolases (GHs). Glycosylation is a fundamental enzymatic process in nature, affecting function of many types of molecules (glycans, proteins, lipids as well as other organic molecules such as the fl avonoids). Possibilities to control glycosylation thus mean possibilities to control or modify the function of the molecule. For the fl avonoids, glycosylation affect both the antioxidative power and solubility. In this chapter we overview results on in vitro deglycosylation and glycosylation of fl avonoids by selected GHs. For optimal enzymatic performance, desired features include a correct specificity for the target, combined with high stability. Poor specifi city towards a specifi c substituent is thus a major drawback for enzymes in particular applications. Efforts to develop the enzymes as conversion tools are reviewed.

Details

Authors
Organisations
External organisations
  • Lund University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biocatalysis and Enzyme Technology

Keywords

  • Amylase, Cellulase, Flavonoid, Gh, Glucosidase, Glycosynthase, Quercetin
Original languageEnglish
Title of host publicationAdvances in Enzyme Biotechnology
PublisherSpringer India
Pages9-21
Number of pages13
ISBN (Electronic)9788132210948
ISBN (Print)9788132210931
Publication statusPublished - 2013 Jan 1
Publication categoryResearch
Peer-reviewedYes