Grafted biomembranes containing membrane proteins--the case of the leucine transporter

Research output: Contribution to journalArticle

Abstract

Here, we bind the sodium dependent amino acid transporter on nitrilotriacetic acid/polyethylene glycol functionalized gold sensors in detergents and perform a detergent-lipid exchange with phosphatidylcholine. We characterize the LeuT structure in the adsorbed film by magnetic contrast neutron reflection using the predicted model from molecular dynamic simulations.

Details

Authors
  • Vivien Jagalski
  • Robert D Barker
  • Mikkel B Thygesen
  • Kamil Gotfryd
  • Mie B Krüger
  • Lei Shi
  • Selma Maric
  • Nicolas Bovet
  • Martine Moulin
  • Michael Haertlein
  • Thomas Günther Pomorski
  • Claus J Loland
  • Marité Cárdenas
Organisations
External organisations
  • University of Copenhagen
Research areas and keywords

Keywords

  • Amino Acid Transport Systems/chemistry, Detergents/chemistry, Gold/chemistry, Lipid Bilayers/chemistry, Molecular Dynamics Simulation, Nitrilotriacetic Acid/chemistry, Phosphatidylcholines/chemistry, Polyethylene Glycols/chemistry, Quartz Crystal Microbalance Techniques, Sodium/chemistry
Original languageEnglish
Pages (from-to)7707-11
Number of pages5
JournalSoft Matter
Volume11
Issue number39
Publication statusPublished - 2015 Oct 21
Publication categoryResearch
Peer-reviewedYes