Granule docking and cargo release in pancreatic beta-cells

Research output: Contribution to journalArticle

Abstract

Biphasic insulin secretion is required for proper insulin action and is observed not only in vivo, but also in isolated pancreatic islets and even single beta-cells. Late events in the granule life cycle are thought to underlie this temporal pattern. In the last few years, we have therefore combined live cell imaging and electrophysiology to study insulin secretion at the level of individual granules, as they approach the plasma membrane, undergo exocytosis and finally release their insulin cargo. In the present paper, we review evidence for two emerging concepts that affect insulin secretion at the level of individual granules: (i) the existence of specialized sites where granules dock in preparation for exocytosis; and (ii) post-exocytotic regulation of cargo release by the fusion pore.

Details

Authors
  • Sebastian Barg
  • Anders Lindqvist
  • Stefanie Obermüller
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology

Keywords

  • syntaxin, imaging, live-cell, insulin secretory granule, fusion pore, docking, exocytosis
Original languageEnglish
Pages (from-to)294-299
JournalBiochemical Society Transactions
Volume36
Publication statusPublished - 2008
Publication categoryResearch
Peer-reviewedYes