Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype.

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Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype. / Bordería, Antonio V; Isakov, Ofer; Moratorio, Gonzalo; Henningsson, Rasmus; Agüera-González, Sonia; Organtini, Lindsey; Gnädig, Nina F; Blanc, Hervé; Alcover, Andrés; Hafenstein, Susan; Fontes, Magnus; Shomron, Noam; Vignuzzi, Marco.

In: PLoS Pathogens, Vol. 11, No. 5, e1004838, 2015.

Research output: Contribution to journalArticle

Harvard

Bordería, AV, Isakov, O, Moratorio, G, Henningsson, R, Agüera-González, S, Organtini, L, Gnädig, NF, Blanc, H, Alcover, A, Hafenstein, S, Fontes, M, Shomron, N & Vignuzzi, M 2015, 'Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype.', PLoS Pathogens, vol. 11, no. 5, e1004838. https://doi.org/10.1371/journal.ppat.1004838

APA

Bordería, A. V., Isakov, O., Moratorio, G., Henningsson, R., Agüera-González, S., Organtini, L., ... Vignuzzi, M. (2015). Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype. PLoS Pathogens, 11(5), [e1004838]. https://doi.org/10.1371/journal.ppat.1004838

CBE

Bordería AV, Isakov O, Moratorio G, Henningsson R, Agüera-González S, Organtini L, Gnädig NF, Blanc H, Alcover A, Hafenstein S, Fontes M, Shomron N, Vignuzzi M. 2015. Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype. PLoS Pathogens. 11(5). https://doi.org/10.1371/journal.ppat.1004838

MLA

Vancouver

Author

Bordería, Antonio V ; Isakov, Ofer ; Moratorio, Gonzalo ; Henningsson, Rasmus ; Agüera-González, Sonia ; Organtini, Lindsey ; Gnädig, Nina F ; Blanc, Hervé ; Alcover, Andrés ; Hafenstein, Susan ; Fontes, Magnus ; Shomron, Noam ; Vignuzzi, Marco. / Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype. In: PLoS Pathogens. 2015 ; Vol. 11, No. 5.

RIS

TY - JOUR

T1 - Group Selection and Contribution of Minority Variants during Virus Adaptation Determines Virus Fitness and Phenotype.

AU - Bordería, Antonio V

AU - Isakov, Ofer

AU - Moratorio, Gonzalo

AU - Henningsson, Rasmus

AU - Agüera-González, Sonia

AU - Organtini, Lindsey

AU - Gnädig, Nina F

AU - Blanc, Hervé

AU - Alcover, Andrés

AU - Hafenstein, Susan

AU - Fontes, Magnus

AU - Shomron, Noam

AU - Vignuzzi, Marco

PY - 2015

Y1 - 2015

N2 - Understanding how a pathogen colonizes and adapts to a new host environment is a primary aim in studying emerging infectious diseases. Adaptive mutations arise among the thousands of variants generated during RNA virus infection, and identifying these variants will shed light onto how changes in tropism and species jumps can occur. Here, we adapted Coxsackie virus B3 to a highly permissive and less permissive environment. Using deep sequencing and bioinformatics, we identified a multi-step adaptive process to adaptation involving residues in the receptor footprints that correlated with receptor availability and with increase in virus fitness in an environment-specific manner. We show that adaptation occurs by selection of a dominant mutation followed by group selection of minority variants that together, confer the fitness increase observed in the population, rather than selection of a single dominant genotype.

AB - Understanding how a pathogen colonizes and adapts to a new host environment is a primary aim in studying emerging infectious diseases. Adaptive mutations arise among the thousands of variants generated during RNA virus infection, and identifying these variants will shed light onto how changes in tropism and species jumps can occur. Here, we adapted Coxsackie virus B3 to a highly permissive and less permissive environment. Using deep sequencing and bioinformatics, we identified a multi-step adaptive process to adaptation involving residues in the receptor footprints that correlated with receptor availability and with increase in virus fitness in an environment-specific manner. We show that adaptation occurs by selection of a dominant mutation followed by group selection of minority variants that together, confer the fitness increase observed in the population, rather than selection of a single dominant genotype.

U2 - 10.1371/journal.ppat.1004838

DO - 10.1371/journal.ppat.1004838

M3 - Article

VL - 11

JO - PLoS Pathogens

T2 - PLoS Pathogens

JF - PLoS Pathogens

SN - 1553-7366

IS - 5

M1 - e1004838

ER -