Growth and hydrogen production characteristics of Caldicellulosiruptor saccharolyticus on chemically defined minimal media

Research output: Contribution to journalArticle


Caldicellulosiruptor saccharolyticus is an extreme thermophilic bacterium recognized for its saccharolytic ability and superior ability to produce high yields of hydrogen. However,most studies have been made using yeast extract (YE) as a rich but expensive nutrient
source. For the first time, we show that C. saccharolyticus is able to grow on defined minimal media, including essential vitamins, provided that CO2 was allowed to accumulate sufficiently
in the culture broth to activate growth. Growth and hydrogen production performance on minimal media was analyzed in both batch and continuous mode. Absence of YE resulted in similar or higher hydrogen yields and specific hydrogen productivities but lower
volumetric hydrogen productivities than with YE. The results also indicate that YE is used as a carbon- and energy source thus affecting metabolic flux calculations. This study clarified that YE is not essential making C. saccharolyticus more attractive for fundamental studies on its metabolism and future industrial exploitation.


Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology


  • Caldicellulosiruptor saccharolyticus Minimal media Hydrogen production Growth activation CO2 sparging
Original languageEnglish
Pages (from-to)4925-4929
JournalInternational Journal of Hydrogen Energy
Issue number6
Publication statusPublished - 2012
Publication categoryResearch