Guthrie card methylomics identifies temporally stable epialleles that are present at birth in humans

Research output: Contribution to journalArticle

Abstract

A major concern in common disease epigenomics is distinguishing causal from consequential epigenetic variation. One means of addressing this issue is to identify the temporal origins of epigenetic variants via longitudinal analyses. However, prospective birth-cohort studies are expensive and time consuming. Here, we report DNA methylomics of archived Guthrie cards for the retrospective longitudinal analyses of in-utero-derived DNA methylation variation. We first validate two methodologies for generating comprehensive DNA methylomes from Guthrie cards. Then, using an integrated epigenomic/genomic analysis of Guthrie cards and follow-up samplings, we identify interindividual DNA methylation variation that is present both at birth and 3 yr later. These findings suggest that disease-relevant epigenetic variation could be detected at birth, i.e., before overt clinical disease. Guthrie card methylomics offers a potentially powerful and cost-effective strategy for studying the dynamics of interindividual epigenomic variation in a range of common human diseases.

Details

Authors
  • Huriya Beyan
  • Thomas A. Down
  • Sreeram V. Ramagopalan
  • Kristina Uvebrant
  • Anita Ramelius
  • Michelle L. Holland
  • Carolina Gemma
  • Gavin Giovannoni
  • Bernhard O. Boehm
  • George C. Ebers
  • Åke Lernmark
  • Corrado Cilio
  • R. David Leslie
  • Vardhman K. Rakyan
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Genetics
Original languageEnglish
Pages (from-to)2138-2145
JournalGenome Research
Volume22
Issue number11
Publication statusPublished - 2012
Publication categoryResearch
Peer-reviewedYes