H/ACA small RNA dysfunctions in disease reveal key roles for noncoding RNA modifications in hematopoietic stem cell differentiation

Research output: Contribution to journalArticle

Abstract

Noncoding RNAs control critical cellular processes, although their contribution to disease remains largely unexplored. Dyskerin associates with hundreds of H/ACA small RNAs to generate a multitude of functionally distinct ribonucleoproteins (RNPs). The DKC1 gene, encoding dyskerin, is mutated in the multisystem disorder X-linked dyskeratosis congenita (X-DC). A central question is whether DKC1 mutations affect the stability of H/ACA RNPs, including those modifying ribosomal RNA (rRNA). We carried out comprehensive profiling of dyskerin-associated H/ACA RNPs, revealing remarkable heterogeneity in the expression and function of subsets of H/ACA small RNAs in X-DC patient cells. Using a mass spectrometry approach, we uncovered single-nucleotide perturbations in dyskerin-guided rRNA modifications, providing functional readouts of small RNA dysfunction in X-DC. In addition, we identified that, strikingly, the catalytic activity of dyskerin is required for accurate hematopoietic stem cell differentiation. Altogether, these findings reveal that small noncoding RNA dysfunctions may contribute to the pleiotropic manifestation of human disease.

Details

Authors
  • Cristian Bellodi
  • Mary McMahon
  • Adrian Contreras
  • Dayle Juliano
  • Noam Kopmar
  • Tomoka Nakamura
  • David Maltby
  • Alma Burlingame
  • Sharon A Savage
  • Akiko Shimamura
  • Davide Ruggero
Organisations
External organisations
  • University of California System
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medical Genetics

Keywords

  • Cell Cycle Proteins, Cell Differentiation, Dyskeratosis Congenita, Hematopoietic Stem Cells, Humans, Mutation, Nuclear Proteins, RNA, Ribosomal, RNA, Untranslated, Ribonucleoproteins, Small Nucleolar
Original languageEnglish
Pages (from-to)1493-502
Number of pages10
JournalCell Reports
Volume3
Issue number5
Publication statusPublished - 2013 May 30
Publication categoryResearch
Peer-reviewedYes