Haemophilus Protein F Orthologs of Pathogens Infecting the Airways: Exploiting Host Laminin at Heparin-Binding Sites for Maximal Adherence to Epithelial Cells

Research output: Contribution to journalArticle

Abstract

Haemophilus influenzae protein F (PF) is an important virulence factor interacting with laminin, an extracellular matrix protein ubiquitously expressed in the respiratory tract. Here we defined PF orthologs in Pseudomonas aeruginosa, Moraxella catarrhalis, and Staphylococcus aureus, bacteria that occasionally colonize and infect the human airways. Despite low sequence homology (48.2%-77.3% similarity), all orthologs (Paf, AfeA, and MntC) interacted with laminin. Interestingly, all proteins bound at the heparin-binding sites of laminin, including the globular domains, and also attached to laminin expressed on respiratory epithelial cells. Laminin is thus a highly important target for PF orthologs of the bacterial species examined.

Details

Authors
Organisations
External organisations
  • Colzyx AB
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology in the medical area

Keywords

  • Adherence, airway pathogen, epithelial cells, heparin-binding domain, laminin, LG globular domain
Original languageEnglish
Pages (from-to)1303-1307
Number of pages5
JournalThe Journal of infectious diseases
Volume216
Issue number10
Publication statusPublished - 2017
Publication categoryResearch
Peer-reviewedYes