High bioethanol titre from Manihot glaziovii through fed-batch simultaneous saccharification and fermentation in Automatic Gas Potential Test System.

Research output: Contribution to journalArticle


A process for the production of high bioethanol titre was established through fed-batch and simultaneous saccharification and fermentation (FB-SSF) of wild, non-edible cassava Manihot glaziovii. FB-SSF allowed fermentation of up to 390g/L of starch-derived glucose achieving high bioethanol concentration of up to 190g/L (24% v/v) with yields of around 94% of the theoretical value. The wild cassava M. glaziovii starch is hydrolysable with a low dosage of amylolytic enzymes (0.1-0.15% v/w, Termamyl® and AMG®). The Automatic Gas Potential Test System (AMPTS) was adapted to yeast ethanol fermentation and demonstrated to be an accurate, reliable and flexible device for studying the kinetics of yeast in SSF and FB-SSF. The bioethanol derived stoichiometrically from the CO2 registered in the AMPTS software correlated positively with samples analysed by HPLC (R(2)=0.99).


  • Anselm Moshi
  • Carla Crespo
  • Malik Badshah
  • Kenneth M M Hosea
  • Anthony Manoni Mshandete
  • Bo Mattiasson
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology
Original languageEnglish
Pages (from-to)348-356
JournalBioresource Technology
Publication statusPublished - 2014
Publication categoryResearch