High capacity xylose transport in Candida intermedia PYCC4715.

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High capacity xylose transport in Candida intermedia PYCC4715. / Gárdonyi, Márk; Österberg, Måns; Rodrigues, C; Spencer-Martins, I; Hahn-Hägerdal, Bärbel.

In: FEMS Yeast Research, Vol. 3, No. 1, 2003, p. 45-52.

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Gárdonyi, M, Österberg, M, Rodrigues, C, Spencer-Martins, I & Hahn-Hägerdal, B 2003, 'High capacity xylose transport in Candida intermedia PYCC4715.', FEMS Yeast Research, vol. 3, no. 1, pp. 45-52. https://doi.org/10.1016/S1567-1356(02)00137-X

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Gárdonyi, Márk ; Österberg, Måns ; Rodrigues, C ; Spencer-Martins, I ; Hahn-Hägerdal, Bärbel. / High capacity xylose transport in Candida intermedia PYCC4715. In: FEMS Yeast Research. 2003 ; Vol. 3, No. 1. pp. 45-52.

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TY - JOUR

T1 - High capacity xylose transport in Candida intermedia PYCC4715.

AU - Gárdonyi, Márk

AU - Österberg, Måns

AU - Rodrigues, C

AU - Spencer-Martins, I

AU - Hahn-Hägerdal, Bärbel

PY - 2003

Y1 - 2003

N2 - Xylose-utilising yeasts were screened to identify strains with high xylose transport capacity. Among the fastest-growing strains in xylose medium, Candida intermedia PYCC 4715 showed the highest xylose transport capacity. Maximal specific growth rate was the same in glucose and xylose media (small mu, Greekmax=0.5 h−1, 30°C). Xylose transport showed biphasic kinetics when cells were grown in either xylose- or glucose-limited culture. The high-affinity xylose/proton symport system (Km=0.2 mM, Vmax=7.5 mmol h−1 g−1) was more repressed by glucose than by xylose. The less specific low-affinity transport system (K=50 mM, Vmax=11 mmol h−1 g−1) appeared to operate through a facilitated-diffusion mechanism and was expressed constitutively. Inhibition experiments showed that glucose is a substrate of both xylose transport systems.

AB - Xylose-utilising yeasts were screened to identify strains with high xylose transport capacity. Among the fastest-growing strains in xylose medium, Candida intermedia PYCC 4715 showed the highest xylose transport capacity. Maximal specific growth rate was the same in glucose and xylose media (small mu, Greekmax=0.5 h−1, 30°C). Xylose transport showed biphasic kinetics when cells were grown in either xylose- or glucose-limited culture. The high-affinity xylose/proton symport system (Km=0.2 mM, Vmax=7.5 mmol h−1 g−1) was more repressed by glucose than by xylose. The less specific low-affinity transport system (K=50 mM, Vmax=11 mmol h−1 g−1) appeared to operate through a facilitated-diffusion mechanism and was expressed constitutively. Inhibition experiments showed that glucose is a substrate of both xylose transport systems.

U2 - 10.1016/S1567-1356(02)00137-X

DO - 10.1016/S1567-1356(02)00137-X

M3 - Article

VL - 3

SP - 45

EP - 52

JO - FEMS Yeast Research

JF - FEMS Yeast Research

SN - 1567-1364

IS - 1

ER -