High genetic load in an old isolated butterfly population

Research output: Contribution to journalArticle

Abstract

We investigated inbreeding depression and genetic load in a small (Ne ∼ 100) population of the Glanville fritillary butterfly (Melitaea cinxia), which has been completely isolated on a small island [Pikku Tytärsaari (PT)] in the Baltic Sea for at least 75 y. As a reference, we studied conspecific populations from the well-studied metapopulation in the Åland Islands (ÅL), 400 km away. A large population in Saaremaa, Estonia, was used as a reference for estimating genetic diversity and Ne. We investigated 58 traits related to behavior, development, morphology, reproductive performance, and metabolism. The PT population exhibited high genetic load (L = 1 − WPT/WÅL) in a range of fitness-related traits including adult weight (L = 0.12), flight metabolic rate (L = 0.53), egg viability (L = 0.37), and lifetime production of eggs in an outdoor population cage (L = 0.70). These results imply extensive fixation of deleterious recessive mutations, supported by greatly reduced diversity in microsatellite markers and immediate recovery (heterosis) of egg viability and flight metabolic rate in crosses with other populations. There was no significant inbreeding depression in most traits due to one generation of full-sib mating. Resting metabolic rate was significantly elevated in PT males, which may be related to their short lifespan (L = 0.25). The demographic history and the effective size of the PT population place it in the part of the parameter space in which models predict mutation accumulation. This population exemplifies the increasingly common situation in fragmented landscapes, in which small and completely isolated populations are vulnerable to extinction due to high genetic load.

Details

Authors
  • Anniina Mattila
  • Anne Duplouy
  • Malla Kirjokangas
  • Rainer Lehtonen
  • Pasi Rastas
  • Ilkka Hanski
External organisations
  • University of Helsinki
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Ecology
Original languageEnglish
Pages (from-to)E2496-E2505
JournalProceedings of the National Academy of Sciences of the United States of America
Volume109
Issue number37
Publication statusPublished - 2012
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes