High-Resolution Characterization of Organic Phosphorus in Soil Extracts Using 2D H-1-P-31 NMR Correlation Spectroscopy

Research output: Contribution to journalArticle

Standard

High-Resolution Characterization of Organic Phosphorus in Soil Extracts Using 2D H-1-P-31 NMR Correlation Spectroscopy. / Vestergren, Johan; Vincent, Andrea G.; Jansson, Mats; Persson, Per; Istedt, Ulrik; Groebner, Gerhard; Giesler, Reiner; Schleucher, Juergen.

In: Environmental Science & Technology, Vol. 46, 2012, p. 3950-3956.

Research output: Contribution to journalArticle

Harvard

APA

CBE

MLA

Vancouver

Author

Vestergren, Johan ; Vincent, Andrea G. ; Jansson, Mats ; Persson, Per ; Istedt, Ulrik ; Groebner, Gerhard ; Giesler, Reiner ; Schleucher, Juergen. / High-Resolution Characterization of Organic Phosphorus in Soil Extracts Using 2D H-1-P-31 NMR Correlation Spectroscopy. In: Environmental Science & Technology. 2012 ; Vol. 46. pp. 3950-3956.

RIS

TY - JOUR

T1 - High-Resolution Characterization of Organic Phosphorus in Soil Extracts Using 2D H-1-P-31 NMR Correlation Spectroscopy

AU - Vestergren, Johan

AU - Vincent, Andrea G.

AU - Jansson, Mats

AU - Persson, Per

AU - Istedt, Ulrik

AU - Groebner, Gerhard

AU - Giesler, Reiner

AU - Schleucher, Juergen

N1 - 7

PY - 2012

Y1 - 2012

N2 - Organic phosphorus (P) compounds represent a major component of soil P in many soils and are key sources of P for microbes and plants. Solution NMR (nuclear magnetic resonance spectroscopy) is a powerful technique for characterizing organic P species. However, P-31 NMR spectra are often complicated by overlapping peaks, which hampers identification and quantification of the numerous P species present in soils. Overlap is often exacerbated by the presence of paramagnetic metal ions, even if they are in complexes with EDTA following NaOH/EDTA extraction. By removing paramagnetic impurities using a new precipitation protocol, we achieved a dramatic improvement in spectral resolution. Furthermore, the obtained reduction in line widths enabled the use of multidimensional NMR methods to resolve overlapping P-31 signals. Using the new protocol on samples from two boreal humus soils with different Fe contents, 2D H-1-P-31 correlation spectra allowed unambiguous identification of a large number of P species based on their P-31 and H-1 chemical shifts and their characteristic coupling patterns, which would not have been possible using previous protocols. This approach can be used to identify organic P species in samples from both terrestrial and aquatic environments increasing our understanding of organic P biogeochemistry.

AB - Organic phosphorus (P) compounds represent a major component of soil P in many soils and are key sources of P for microbes and plants. Solution NMR (nuclear magnetic resonance spectroscopy) is a powerful technique for characterizing organic P species. However, P-31 NMR spectra are often complicated by overlapping peaks, which hampers identification and quantification of the numerous P species present in soils. Overlap is often exacerbated by the presence of paramagnetic metal ions, even if they are in complexes with EDTA following NaOH/EDTA extraction. By removing paramagnetic impurities using a new precipitation protocol, we achieved a dramatic improvement in spectral resolution. Furthermore, the obtained reduction in line widths enabled the use of multidimensional NMR methods to resolve overlapping P-31 signals. Using the new protocol on samples from two boreal humus soils with different Fe contents, 2D H-1-P-31 correlation spectra allowed unambiguous identification of a large number of P species based on their P-31 and H-1 chemical shifts and their characteristic coupling patterns, which would not have been possible using previous protocols. This approach can be used to identify organic P species in samples from both terrestrial and aquatic environments increasing our understanding of organic P biogeochemistry.

U2 - 10.1021/es204016h

DO - 10.1021/es204016h

M3 - Article

VL - 46

SP - 3950

EP - 3956

JO - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 1520-5851

ER -