High-Resolution Genotyping of Chlamydia trachomatis Strains by Multilocus Sequence Analysis.

Research output: Contribution to journalArticle

Abstract

Genotyping of Chlamydia trachomatis is limited by the low sequence variation in the genome, and no adequate method is available for analysis of the spread of chlamydial infections in the community. We have developed a multillocus sequence typing (MLST) system based on five target regions and compared it with analysis of ompA, the single gene most extensively used for genotyping. Sequence determination of 16 reference strains, comprising all major serotypes, serotypes A to L3, showed that the number of genetic variants in the five separate target regions ranged from 8 to 16. The genetic variation in 47 clinical C. trachomatis isolates of representative serotypes (14 serotype D, 12 serotype E, 11 serotype G, and 10 serotype K strains) was analyzed; and the MLST system detected 32 variants, whereas 12 variants were detected by using ompA analysis. Specimens of the predominant serotype, serotype E, were differentiated into seven genotypes by MLST but into only two by ompA analysis. The MLST system was applied to C. trachomatis specimens from a population of men who have sex with men and was able to differentiate 10 specimens of one predominant ompA genotype G variant into four distinct MLST variants. To conclude, our MLST system can be used to discriminate C. trachomatis strains and can be applied to high-resolution molecular epidemiology.

Details

Authors
  • Markus Klint
  • Hans-Henrik Fuxelius
  • Renée Rostlinger Goldkuhl
  • Hanna Skarin
  • Christian Rutemark
  • Siv G E Andersson
  • Kenneth Persson
  • Bjorn Herrmann
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Microbiology in the medical area
Original languageEnglish
Pages (from-to)1410-1414
JournalJournal of Clinical Microbiology
Volume45
Issue number5
Publication statusPublished - 2007
Publication categoryResearch
Peer-reviewedYes