Hormone-sensitive lipase is a cholesterol esterase of the intestinal mucosa

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Hormone-sensitive lipase is a cholesterol esterase of the intestinal mucosa. / Grober, J; Lucas, S; Sörhede Winzell, Maria; Zaghini, I; Mairal, A; Contreras, Juan Antonio; Besnard, P; Holm, Cecilia; Langin, D.

In: Journal of Biological Chemistry, Vol. 278, No. 8, 2003, p. 6510-6515.

Research output: Contribution to journalArticle

Harvard

Grober, J, Lucas, S, Sörhede Winzell, M, Zaghini, I, Mairal, A, Contreras, JA, Besnard, P, Holm, C & Langin, D 2003, 'Hormone-sensitive lipase is a cholesterol esterase of the intestinal mucosa', Journal of Biological Chemistry, vol. 278, no. 8, pp. 6510-6515. https://doi.org/10.1074/jbc.M208513200

APA

Grober, J., Lucas, S., Sörhede Winzell, M., Zaghini, I., Mairal, A., Contreras, J. A., Besnard, P., Holm, C., & Langin, D. (2003). Hormone-sensitive lipase is a cholesterol esterase of the intestinal mucosa. Journal of Biological Chemistry, 278(8), 6510-6515. https://doi.org/10.1074/jbc.M208513200

CBE

Grober J, Lucas S, Sörhede Winzell M, Zaghini I, Mairal A, Contreras JA, Besnard P, Holm C, Langin D. 2003. Hormone-sensitive lipase is a cholesterol esterase of the intestinal mucosa. Journal of Biological Chemistry. 278(8):6510-6515. https://doi.org/10.1074/jbc.M208513200

MLA

Vancouver

Grober J, Lucas S, Sörhede Winzell M, Zaghini I, Mairal A, Contreras JA et al. Hormone-sensitive lipase is a cholesterol esterase of the intestinal mucosa. Journal of Biological Chemistry. 2003;278(8):6510-6515. https://doi.org/10.1074/jbc.M208513200

Author

Grober, J ; Lucas, S ; Sörhede Winzell, Maria ; Zaghini, I ; Mairal, A ; Contreras, Juan Antonio ; Besnard, P ; Holm, Cecilia ; Langin, D. / Hormone-sensitive lipase is a cholesterol esterase of the intestinal mucosa. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 8. pp. 6510-6515.

RIS

TY - JOUR

T1 - Hormone-sensitive lipase is a cholesterol esterase of the intestinal mucosa

AU - Grober, J

AU - Lucas, S

AU - Sörhede Winzell, Maria

AU - Zaghini, I

AU - Mairal, A

AU - Contreras, Juan Antonio

AU - Besnard, P

AU - Holm, Cecilia

AU - Langin, D

PY - 2003

Y1 - 2003

N2 - The identity of the enzymes responsible for lipase and cholesterol esterase activities in the small intestinal mucosa is not known. Because hormone-sensitive lipase (HSL) catalyzes the hydrolysis of acylglycerols and cholesteryl esters, we sought to determine whether HSL could be involved. HSL mRNA and protein were detected in all segments of the small intestine by Northern and Western blot analyses, respectively. Immunocytochemistry experiments revealed that HSL was expressed in the differentiated enterocytes of the villi and was absent in the undifferentiated cells of the crypt. Diacylglycerol lipase and cholesterol esterase activities were found in the different segments. Analysis of gut from HSL-null mice showed that diacylglycerol lipase activity was unchanged in the duodenum and reduced in jejunum. Neutral cholesterol esterase activity was totally abolished in duodenum, jejunum, and ileum of HSL-null mice. Analysis of HSL mRNA structure showed two types of transcripts expressed in equal amounts with alternative 5'-ends transcribed from two exons. This work demonstrates that HSL is expressed in the mucosa of the small intestine. The results also reveal that the enzyme participates in acylglycerol hydrolysis in jejunal enterocytes and cholesteryl ester hydrolysis throughout the small intestine.

AB - The identity of the enzymes responsible for lipase and cholesterol esterase activities in the small intestinal mucosa is not known. Because hormone-sensitive lipase (HSL) catalyzes the hydrolysis of acylglycerols and cholesteryl esters, we sought to determine whether HSL could be involved. HSL mRNA and protein were detected in all segments of the small intestine by Northern and Western blot analyses, respectively. Immunocytochemistry experiments revealed that HSL was expressed in the differentiated enterocytes of the villi and was absent in the undifferentiated cells of the crypt. Diacylglycerol lipase and cholesterol esterase activities were found in the different segments. Analysis of gut from HSL-null mice showed that diacylglycerol lipase activity was unchanged in the duodenum and reduced in jejunum. Neutral cholesterol esterase activity was totally abolished in duodenum, jejunum, and ileum of HSL-null mice. Analysis of HSL mRNA structure showed two types of transcripts expressed in equal amounts with alternative 5'-ends transcribed from two exons. This work demonstrates that HSL is expressed in the mucosa of the small intestine. The results also reveal that the enzyme participates in acylglycerol hydrolysis in jejunal enterocytes and cholesteryl ester hydrolysis throughout the small intestine.

U2 - 10.1074/jbc.M208513200

DO - 10.1074/jbc.M208513200

M3 - Article

VL - 278

SP - 6510

EP - 6515

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 1083-351X

IS - 8

ER -