Human adipogenesis is associated with genome-wide DNA methylation and gene-expression changes

Research output: Contribution to journalArticle

Abstract

Aim: To define the genomic distribution and function of DNA methylation changes during human adipogenesis. Methods: We isolated adipocyte-derived stem cells from 13 individuals and analyzed genome-wide DNA methylation and gene expression in cultured adipocyte-derived stem cells and mature adipocytes. Results: We observed altered DNA methylation of 11,947 CpG sites and altered expression of 11,830 transcripts after differentiation. De novo methylation was observed across all genomic elements. Co-existence of genes with both altered expression and DNA methylation was found in genes important for cell cycle and adipokine signaling. Conclusion: Human adipogenesis is associated with significant DNA methylation changes across the entire genome and may impact regulation of cell cycle and adipokine signaling.

Details

Authors
Organisations
External organisations
  • Copenhagen University Hospital
  • Steno Diabetes Center Copenhagen
  • University of Copenhagen
  • Skåne University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Endocrinology and Diabetes
  • Medical Genetics

Keywords

  • adipogenesis, adiponectin, DNA methylation, epigenetics, stem cells
Original languageEnglish
Pages (from-to)1601-1617
Number of pages17
JournalEpigenomics
Volume8
Issue number12
Publication statusPublished - 2016 Dec 1
Publication categoryResearch
Peer-reviewedYes