Human immunoglobulin class and subclass specificity of Fc receptors induced by herpes simplex virus type 1

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Human immunoglobulin class and subclass specificity of Fc receptors induced by herpes simplex virus type 1. / Johansson, Hugo; Hallberg, Torgny; Oxelius, Vivi-Anne; Grubb, Anders; Blomberg, Jonas.

In: Journal of Virology, Vol. 50, No. 3, 1984, p. 796-804.

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Johansson, Hugo ; Hallberg, Torgny ; Oxelius, Vivi-Anne ; Grubb, Anders ; Blomberg, Jonas. / Human immunoglobulin class and subclass specificity of Fc receptors induced by herpes simplex virus type 1. In: Journal of Virology. 1984 ; Vol. 50, No. 3. pp. 796-804.

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TY - JOUR

T1 - Human immunoglobulin class and subclass specificity of Fc receptors induced by herpes simplex virus type 1

AU - Johansson, Hugo

AU - Hallberg, Torgny

AU - Oxelius, Vivi-Anne

AU - Grubb, Anders

AU - Blomberg, Jonas

PY - 1984

Y1 - 1984

N2 - Herpes simplex virus is known to induce an immunoglobulin-binding cell surface receptor in infected cells that utilizes a nonimmune mechanism. In the present paper, we report the immunoglobulin class and subclass specificity of this receptor. Of the human immunoglobulins G(IgG), IgA, IgM, and IgD, as well as the structurally related beta2 microglobulin, only IgG and its Fc portion exhibited an increased binding to herpes simplex virus-infected cells versus uninfected control cells. The IgG subclass specificity of the Fc receptor was studied in 37 radioiodinated IgG myeloma proteins representing all four subclasses. We found that IgG3 myeloma proteins did not bind to herpes simplex virus-infected cells to a greater extent than to uninfected cells. On the contrary, proteins belonging to the other subclasses exhibited an increased binding to herpes simplex virus-infected cells of the following relative magnitude: IgG4 greater than IgG1 greater than or equal to IgG2. This increment of binding could be abolished by addition of a large excess of human IgG Fc fragment. Evidence for the existence of a variable herpes simplex virus-specific binding ability between myeloma proteins belonging to the same IgG subclass was also obtained. Furthermore, we tested two other herpes simplex virus type 1 strains with a limited number of myeloma proteins with very similar results as with the herpes simplex virus type 1 F strain. Several sources of experimental artefacts were controlled, including the state of aggregation of the test proteins, the functional integrity of the Fc portion before and after radioiodination, and the subclass assignments. The implications for the biological role of the Fc receptor of herpes simplex virus are discussed.

AB - Herpes simplex virus is known to induce an immunoglobulin-binding cell surface receptor in infected cells that utilizes a nonimmune mechanism. In the present paper, we report the immunoglobulin class and subclass specificity of this receptor. Of the human immunoglobulins G(IgG), IgA, IgM, and IgD, as well as the structurally related beta2 microglobulin, only IgG and its Fc portion exhibited an increased binding to herpes simplex virus-infected cells versus uninfected control cells. The IgG subclass specificity of the Fc receptor was studied in 37 radioiodinated IgG myeloma proteins representing all four subclasses. We found that IgG3 myeloma proteins did not bind to herpes simplex virus-infected cells to a greater extent than to uninfected cells. On the contrary, proteins belonging to the other subclasses exhibited an increased binding to herpes simplex virus-infected cells of the following relative magnitude: IgG4 greater than IgG1 greater than or equal to IgG2. This increment of binding could be abolished by addition of a large excess of human IgG Fc fragment. Evidence for the existence of a variable herpes simplex virus-specific binding ability between myeloma proteins belonging to the same IgG subclass was also obtained. Furthermore, we tested two other herpes simplex virus type 1 strains with a limited number of myeloma proteins with very similar results as with the herpes simplex virus type 1 F strain. Several sources of experimental artefacts were controlled, including the state of aggregation of the test proteins, the functional integrity of the Fc portion before and after radioiodination, and the subclass assignments. The implications for the biological role of the Fc receptor of herpes simplex virus are discussed.

M3 - Article

VL - 50

SP - 796

EP - 804

JO - Journal of Virology

JF - Journal of Virology

SN - 1098-5514

IS - 3

ER -