Human MoAbs produced from normal, HIV-1-negative donors and specific for glycoprotein gp120 of the HIV-1 envelope

Research output: Contribution to journalArticle

Abstract

Human MoAbs ofIgM class were developed against three regions of the HIV-1 envelope. Uninfected donor lymphocytes were immunized in vitro with recombinant protein pB1. Four out of five antibodies were directed to different parts of the V3 region, which contains a major neutralizing site. Two out of these antibodies were directed to more than one amino acid sequence, indicating reactivity to discontinuous sites. Two of the human MoAbs inhibited viral spread between cells in tissue culture, interpreted as reactivities to conserved amino acid sequences exposed during viral maturation. No MoAb neutralized virus, which may be explained by the relatively low avidity of the antibodies. One MoAb was directed to a region containing amino acids participating in CD4 binding. This technique appears to allow formation of antibodies with fine specificities other than those obtained in infected hosts.

Details

Authors
Organisations
External organisations
  • National Bacteriological Laboratory, Stockholm
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area

Keywords

  • gp120, HIV-1, human MoAbs, in vitro immunization, V3 region
Original languageEnglish
Pages (from-to)290-295
Number of pages6
JournalClinical and Experimental Immunology
Volume89
Issue number2
Publication statusPublished - 1992
Publication categoryResearch
Peer-reviewedYes