Humoral immunity targeting site I of antigenic domain 2 of glycoprotein B upon immunization with different cytomegalovirus candidate vaccines.

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Abstract

Glycoprotein B (gB) is a major component in several vaccines that are under development for prevention of disease by cytomegalovirus. It contains multiple determinants that are targets for neutralizing antibodies. One of them is site I of antigenic domain 2 (AD-2). The epitope, defined by short peptides, is quite conserved between different isolates. However, it is poorly immunogenic in natural infection. In this study we investigated the extent to which different vaccines, attenuated live Towne vaccine with or without priming with a canarypox virus coding for gB, or a recombinant gB vaccine adjuvanted with MF59, induced antibodies to this epitope. As in natural infection only a fraction of all subjects developed antibody responses against site I of AD-2 following vaccination. We suggest that strategies that enhance immunogenicity of this epitope will improve vaccine efficacy.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area

Keywords

  • Viral/blood, Cytomegalovirus Vaccines/immunology, Epitope Mapping, Humans, Immunization, Protein Structure, Antibodies, Tertiary, Vaccines, Attenuated/immunology, Synthetic/immunology, Viral Envelope Proteins/chemistry, Viral Envelope Proteins/immunology
Original languageEnglish
Pages (from-to)41-46
JournalVaccine
Volume26
Issue number1
Publication statusPublished - 2007
Publication categoryResearch
Peer-reviewedYes