Hunting for Protein Markers of Hypoxia by Combining Plasma Membrane Enrichment with a New Approach to Membrane Protein Analysis

Research output: Contribution to journalArticle

Abstract

Nontransient hypoxia is strongly associated with malignant lesions, resulting in aggressive behavior and resistance to treatment. We present an analysis of mRNA and protein expression changes in neuroblastoma cell lines occurring upon the transition from normoxia to hypoxia. The correlation between mRNA and protein level changes was poor, although some known hypoxia-driven genes and proteins correlated well. We present previously undescribed membrane proteins expressed under hypoxic conditions that are candidates for evaluation as biomarkers.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biophysics
  • Immunology in the medical area

Keywords

  • isotopic labeling, HPLC-MS, 2D-PAGE, plasma membrane, protein, expression, mRNA expression, correlation, hypoxia, normoxia, neuroblastoma
Original languageEnglish
Pages (from-to)1645-1656
JournalJournal of Proteome Research
Volume10
Issue number4
Publication statusPublished - 2011
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Computational biology and biological physics (000006113), Molecular Medicine (013031200), Department of Immunotechnology (011029300), Division of Translational Cancer Research (013250500)