Hybrid Microdevice Electrophoretic of Peptides, Proteins, DNA, Viruses and Bacteria in Various Separations Media Using UV-Detection

Research output: Contribution to journalArticle

Abstract

Recently we described the design of a hybrid microdevice for micro(nano)electrophoresis and electrochromatography, discussed its advantages and disadvantages compared to conventional microdevices and presented a few applications with lowmolecular-weight samples. In this paper, we demonstrate the broad application range of this device using UV-based analyses of (i) peptides by free-zone electrophoresis and
electrophoresis in a recently introduced gel (polyacrylamide cross-linked with allyl-b-cyclodextrin), (ii) proteins by electrophoretic molecular-sieving in a polymer solution supplemented with SDS, (iii) DNA fragments by electrophoresis in the above gel, (iv) virus particles in this gel, as well as in free buffer and (v) bacteria in free buffer. To illustrate the advantages of the hybrid microdevice we can mention that electrophoresis of proteins in a polymer-containing buffer, supplemented with sodium dodecyl
sulfate (SDS), in a 4.30 (2.75) cm long channel gave a resolution similar to that in conventional capillary electrophoresis in a 23.5 (18.6) cm long capillary and analysis times which were 15-fold shorter.

Details

Authors
  • Ákos Végvári
  • Stellan Hjertén
External organisations
  • External Organization - Unknown
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Medical Engineering

Keywords

  • Bacteria, DNA, Free-zone electrophoresis, Gel electrophoresis, Microdevice chip, Miniaturization, Peptides, Proteins, Virus
Original languageEnglish
Pages (from-to)3815-3820
JournalElectrophoresis
Volume24
Issue number21
Publication statusPublished - 2003
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes