Hydrodynamic trapping of molecules in lipid bilayers

Research output: Contribution to journalArticle

Abstract

In this work we show how hydrodynamic forces can be used to locally trap molecules in a supported lipid bilayer (SLB). The method uses the hydrodynamic drag forces arising from a flow through a conical pipette with a tip radius of 1-1.5 mu m, placed approximately 1 mu m above the investigated SLB. This results in a localized force-field that acts on molecules protruding from the SLB, yielding a hydrodynamic trap with a size approximately given by the size of the pipette tip. We demonstrate this concept by trapping the protein streptavidin, bound to biotin receptors in the SLB. It is also shown how static and kinetic information about the intermolecular interactions in the lipid bilayer can be obtained by relating how the magnitude of the hydrodynamic forces affects the accumulation of protein molecules in the trap.

Details

Authors
Organisations
External organisations
  • University of Cambridge
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Physical Chemistry

Keywords

  • intermolecular forces, membrane proteins, mobility, non-contact, tweezers, ion conductance microscopy
Original languageEnglish
Pages (from-to)10328-10333
JournalProceedings of the National Academy of Sciences
Volume109
Issue number26
Publication statusPublished - 2012
Publication categoryResearch
Peer-reviewedYes